81,861 results
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Plasmid#164460PurposeLocalization of mGreenLantern fluorescent protein to tubulinDepositorInsertmGreenLantern
TagsTubulinExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_MCP-RecT
Plasmid#164803Purposemammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLSDepositorInsertRecT
ExpressionMammalianAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcNDA-NES-PhoCl2c-mCherry
Plasmid#164037PurposeOptogenetic control of protein localization by PhoCl2cDepositorInsertNES-PhoCl2c-mCherry
Tagskozak sequenceExpressionMammalianPromoterCMV promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-PH-FAPP1
Plasmid#161986PurposeExpresses GFP-tagged PH-FAPP1. PI(4)P biosensorDepositorAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DAAO
Plasmid#60344PurposeYeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.DepositorInsertDAAO
ExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpG
Plasmid#159978PurposePrime editing in mammalian cellsDepositorInsertPE2-SpG
TagsBpSV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-mTagBFP2
Plasmid#161790Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; BicistronicTagsmTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbC and UbC promoterAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMNb-cALFA
Plasmid#159991PurposeFor the protein expression with ALFA tag at C-terminus in mammalian cellsDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationCMV promoter, WPREAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEJS1089: mini-AAV.sgRNA.Nme2Cas9
Plasmid#159536PurposeDelivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 KbDepositorInsertNme2Cas9 with single guide RNA cassette
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCitrine
Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
TagsmCitrineExpressionMammalianAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF1
Plasmid#155346PurposeExpress full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cellsDepositorAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaQ-RLuc8
Plasmid#140982PurposeEncodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaQ-RLuc8 (GNAQ Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/Strep-HA-ZAK alpha
Plasmid#141193PurposeExpresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell lineDepositorAvailable SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV MCS mCherry-KASH
Plasmid#139654PurposeCloning template for AAV-based ORANGE knock-in constructsDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoterU6Available SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF
Plasmid#131710PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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PCR4-Hsp68::lacZ-H11
Plasmid#139099PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingExpressionMammalianPromoterHsp68Available SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHTNW
Plasmid#136403PurposeExpression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.DepositorTypeEmpty backboneTagsHaloTagExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3-WPRE
Plasmid#132318Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I
Plasmid#129721PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mScarlet-IExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only