80,104 results

Showing: 10061 - 10080 of 80104 results

Results per page:

Sort results by:

pFX-EGFP-Rab5

Plasmid

#174454

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
YAP1-V5 in pLX304

Plasmid

#42555

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2873

Plasmid

#143648

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ELF1 (ELF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-DN-hCUL3-FLAG

Plasmid

#15820

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin3 (CUL3 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N418

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-mEGFP

Plasmid

#182876

Purpose

Localization: Endoplasmic Reticulum

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

ER-mEGFP

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmTurquoise2-ER

Plasmid

#36204

Purpose

In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

ER-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI HA NEDD4L DD

Plasmid

#27001

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Mutation

C962A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TSHR-Tango

Plasmid

#66518

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

TSHR (TSHR Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_SpCas9_USP7 Exon 3 gRNA

Plasmid

#131257

Purpose

pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

humanised S. pyogenes Cas9 nuclease

Expression

Mammalian

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PE1

Plasmid

#132774

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE1

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-PIG-METTL14-WT

Plasmid

#141118

Purpose

Retroviral overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-GABARAP G116A

Plasmid

#123098

Purpose

Expresses 3xFLAG-GABARAP G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-WT-Full-length

Plasmid

#194548

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

Plasmid

#141379

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEGFP Sec22b

Plasmid

#101918

Purpose

Expresses Sec22b in mammalian cells

Depositor

Thierry Galli

Article

Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. do

Insert

Sec22b (Sec22b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-N1-EXO1b

Plasmid

#111621

Purpose

Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

Depositor

Marco Muzi-Falconi

Article

Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

Insert

Exonuclease 1b (EXO1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CRHR2-Tango

Plasmid

#66257

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CRHR2 (CRHR2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
mAzurite-16xRhoBAST

Plasmid

#196347

Purpose

Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

mAzurite-16xRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge