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pCW57.1 _Gata6

Plasmid

#73537

Purpose

Inducible lentiviral expression of Gata6

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Gata6 (Gata6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV mTagBFP2-2A-CHMP2B

Plasmid

#232000

Purpose

Bicistronic expression of CHMP2B along with an mTagBFP2 transfection marker, connected via 2A sequences.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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HBx

Plasmid

#65463

Purpose

expresses HBx in mammalian cells

Depositor

Wang-Shick Ryu

Article

Cha et al Hepatology. 2004 Jun;39(6):1683-93.

Insert

HBV X protein

Tags

Flag

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

Plasmid

#162379

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2

Plasmid

#149517

Purpose

N-termial APEX2 tag. Gateway destination vector for mammanlian expression

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Type

Empty backbone

Tags

FLAG-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 NFAT3

Plasmid

#10961

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

NFAT3 (NFATC4 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-P4Mx1

Plasmid

#108121

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-BsaIx2-DD-Cas9

Plasmid

#75380

Purpose

Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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gh40

Plasmid

#106714

Purpose

expression of gRNA targeting ST6GAL1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GAL1 (ST6GAL1 Human)

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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mNeongreen-PXN

Plasmid

#129604

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Paxillin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-LaminB1-10

Plasmid

#54140

Purpose

Localization: Nuclear Envelope, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

LaminB1 (LMNB1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pFX-mito-mKate2

Plasmid

#174178

Purpose

Express mKate2 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mKate2 with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF2242

Plasmid

#141986

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-CAG-Ftractin-GFP

Plasmid

#219569

Purpose

Lentiviral construct expressing GFP-tagged F-tractin

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

F-tractin (Itpka )

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-BFP-NLS-P2A-RSV P-1xALFATag@72AA

Plasmid

#246864

Purpose

Express BFP-NLS-P2A-RSV P-1xALFATag@72AA (Pexo-ALFATag) for RSV imaging.

Depositor

Marvin Tanenbaum

Article

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

BFP-NLS-P2A-RSV P-1xALFATag@72AA

Use

Lentiviral

Tags

BFP-NLS

Expression

Mammalian

Mutation

RSV phosphoprotein (P) contains a single ALFA Tag…

Promoter

SFFV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-DARPin-P-BFP

Plasmid

#246865

Purpose

Express DARPin-P-BFP for RSV imaging.

Depositor

Marvin Tanenbaum

Article

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

DARPin-P (clone ID_011-1055-C6-2605-H5)

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a eIL15 IRES mNeonGreen-P2A-Puro

Plasmid

#248036

Purpose

Constitutively expresses an engineered version of IL15, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

Engineered IL15

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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L4276 hproTGFb1 in pcDNA backbone

Plasmid

#244183

Purpose

Constitutive expression of human pro-TGF-beta1 (with its signal sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

pro-TGF-beta1 (TGFB1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLD1112 TRE-NLS-mMaroon1-NLS-22Xm6A MS2

Plasmid

#235129

Purpose

mMaroon1 reporter plasmid for live cell visualization of RNA m6A modification

Depositor

Qin Peng

Article

Li et al Sci Adv. 2024 Nov 15;10(46):eadp5689. do

Insert

TRE-NLS-mMaroon1-NLS-22Xm6A MS2

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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piRFP_SeV_Phosphoprotein

Plasmid

#228807

Purpose

Expression of Sendai virus Phosphoprotein

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

Phosphoprotein

Tags

iRFP670

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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