78,133 results

1. pLKO-FOXM1-shRNA-81

   Plasmid

   #89495

   Purpose

   Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

   Depositor

   Adam Karpf

   Insert

   FOXM1 (FOXM1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CLuc-FKBP

   Plasmid

   #31184

   Depositor

   David Piwnica-Worms

   Article

   Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

   Insert

   C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-mRipk3

   Plasmid

   #78805

   Purpose

   To overexpress mRipk3 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-N-Flag-HA-IRES-PURO

   Plasmid

   #41033

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Type

   Empty backbone

   Use

   Retroviral; Gateway destination vector

   Tags

   Flag, HA, and IRES Puro

   Expression

   Mammalian

   Promoter

   LTR-driven expression

   Available Since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. p40PX-EGFP

   Plasmid

   #19010

   Depositor

   Michael Yaffe

   Article

   Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

   Insert

   p40phox (NCF4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa1-148 (PX domains)

   Promoter

   CMV

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-PTEN-shRNA-3001

   Plasmid

   #25639

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   PTEN-shRNA-3001 (PTEN Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVTHM-mSox2-3xflag

   Plasmid

   #206376

   Purpose

   Expresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

   Plasmid

   #214994

   Purpose

   Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti SYN-dCas9-KRAB-MeCP2

    Plasmid

    #155365

    Purpose

    Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    3x FLAG and 7x His

    Expression

    Mammalian

    Promoter

    SYN

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-HypaCas9-AAVS1_sgRNA

    Plasmid

    #183891

    Purpose

    pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEnt L1L3 EF1a-tTA-2

    Plasmid

    #26803

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Elongation Factor 1 alpha promoter and tTA transgene
    

    Use

    Gateway entry vector

    Expression

    Mammalian

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcdna3.1 hygro mCherry

    Plasmid

    #128057

    Purpose

    Mammalian expression vector with N terminal mCherry

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. UKNP1.9.1

    Plasmid

    #97374

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. STARR-seq luciferase validation vector_ORI_neg.cont

    Plasmid

    #99315

    Purpose

    Negative control for luciferase validation vectors

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    neutral region; chr3: 55198740-55200041
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIX403_deltaUBA_UBAP2L_mCherry

    Plasmid

    #105287

    Purpose

    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    UBAP2L (UBAP2L Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 20-108

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH-EFIRES-P-Seipin-miniIAA7-mCherry

    Plasmid

    #129720

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mCherry

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3036

    Plasmid

    #144512

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

    Plasmid

    #127332

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mTagBFP2-Rab5a-7

    Plasmid

    #55322

    Purpose

    Localization: Ras GTPase, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Rab5a
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits