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Plasmid#215334PurposeISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI siteDepositorInsertRice codon optimized ISDra2 TnpB
UseTagsNot applicableExpressionPlantMutationPromoterRice ubiquitin promoter for TnpB and maize ubiqui…Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAD7-crRNA-2
Plasmid#170135PurposeFor crRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMPE-ePPEplus
Plasmid#205242PurposeFor multiplex plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRV1
Plasmid#180515PurposeAgrobacterium tumefaciens-based expression of TRV1DepositorInsertTobacco rattle virus RNA1
UseTagsExpressionPlantMutationPromoter35S CaMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX-PVX
Plasmid#213040PurposePVX vector to express heterologous sequences in plantsDepositorInsertPotato virus X
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p35S_GFP-GUS
Plasmid#167122PurposePlant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.DepositorInsertGFP-GUS
UseTagsmGFP5, GUSExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT3T4
Plasmid#50592PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT3, gRNA scaffold, U6-1tplus, U6-26p, T4
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25F
Plasmid#91143PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
35S-eGFP-nosT
Plasmid#80127PurposeTransient expression vector for eGFP in plantsDepositorTypeEmpty backboneUseTagseGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pROF417
Plasmid#155335PurposeConstitutive expression LFY-VP16-2A-RLuc in plantsDepositorInsertPCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E2
Plasmid#164719PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH114
Plasmid#128408PurposeLevel1 ZmUBIp::BAR:NOStDepositorInsertLevel1 ZmUBIp::BAR:NOSt (Position 1)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_220
Plasmid#91113PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRA109
Plasmid#209342PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mTagBFP2 fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL850
Plasmid#214250PurposeExpression TadCBEa in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p32Lb
Plasmid#158154PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
nCas9-PPE
Plasmid#140445PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSKI015
Plasmid#11570DepositorInsertCaMV 35S enhancers
UseActivation taggingTagsExpressionPlantMutationPromoterAvailable sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSV40-mCherry-P2A-Hygro
Plasmid#106478PurposeExpression of mCherry and Hygromycin resistance gene from SV40 promoterDepositorInsertsmCherry
Hygromycin B resistance
UseTagsExpressionMammalian and PlantMutationPromoterSV40 promoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only