1,748 results

1. pJF6

   Plasmid

   #139040

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   gfp (includes STOP codon, PATC introns)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKS235

   Plasmid

   #191855

   Purpose

   Negative selection marker for MosSCI

   Depositor

   K Subramaniam

   Article

   Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

   Insert

   peel-1 toxic protein for negative selection against extrachromosomal array
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.41

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ChrII_ttTi5605_loxP_myo2_neoR

   Plasmid

   #194056

   Purpose

   Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ttTi5605
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSR49

   Plasmid

   #69259

   Purpose

   phlh-8::tagBFP2

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   TagBFP2
   

   Use

   Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0, TagBFP2 contains 2 sing…

   Promoter

   hlh-8

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJJR81

   Plasmid

   #75029

   Purpose

   tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Insert

   tagBFP2^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized tagBFP2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ChrV_oxTi365_loxP_myo2_neoR

   Plasmid

   #194057

   Purpose

   Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   oxTi365
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAS1-lim-4P::his-24::Cerulean

   Plasmid

   #124336

   Purpose

   Multicolor Worms for Neuronal ID

   Depositor

   Andrew Leifer

   Insert

   lim-4, Cerulean (lim-4 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJW2341

   Plasmid

   #186950

   Purpose

   30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

   Depositor

   Jordan Ward

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAB16 (pmyo-3::QuasAr)

   Plasmid

   #130272

   Purpose

   Expresses the voltage sensor QuasAr in BWMs of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

   Insert

   QuasAr
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PU6::klp-12_sgRNA

    Plasmid

    #46170

    Purpose

    klp-12 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    klp-12 targeting sgRNA (klp-12 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

    Plasmid

    #66753

    Purpose

    miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

    Depositor

    Yishi Jin

    Article

    Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    miniSOG
    

    Use

    Tags

    tomm-20 (N terminal 55 aa)

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

    Plasmid

    #159798

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMLS716

    Plasmid

    #188888

    Purpose

    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    floxed Cbr-unc-119 marker and Phsp16.41::Cre
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ108 - pENTR221 - [cbr-unc-119(+)]

    Plasmid

    #200367

    Purpose

    cbr-unc-119 rescue gene for use as co-injection marker.

    Depositor

    Christian Froekjaer-Jensen

    Article

    AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

    Insert

    [cbr-unc-119(+)]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159813

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJH127

    Plasmid

    #162483

    Purpose

    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

    Depositor

    Bob Goldstein

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    

    Use

    CRISPR

    Tags

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRB1080

    Plasmid

    #71309

    Purpose

    Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

    Depositor

    Andrew Fire

    Article

    Bell et al Genetics. 2015 Dec 17. pii: genetics.115

    Insert

    VQR Cas9 variant
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ150-set2

    Plasmid

    #82546

    Purpose

    Pmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::set-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHygCG2r

    Plasmid

    #200074

    Purpose

    hygR selection mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge