8,043 results

1. pDS297

   Plasmid

   #34986

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   cpPDZ
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

   Plasmid

   #68274

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7DS NLS mCherry
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   pRPS2

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Ube2h

   Plasmid

   #31419

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBE2H Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   *see comments

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. Ube2L3

   Plasmid

   #31421

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBE2L3 Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. YIp211-P30-His-pol30(K127R)-3'

   Plasmid

   #99547

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K127R

   Promoter

   POL30

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNXRVat-HACre

   Plasmid

   #78399

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   TKanAX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNXRVbt-HACre

   Plasmid

   #78402

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   HyTkAX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GTL-ir

   Plasmid

   #81102

   Purpose

   Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   tdiRFP
   

   Use

   Cre/Lox

   Tags

   tdiRFP

   Expression

   Yeast

   Mutation

   codon optimized second iRFP to avoid internal rec…

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GTT-y

   Plasmid

   #81110

   Purpose

   Gene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   mCitrine
   

   Use

   Cre/Lox

   Tags

   mCitrine

   Expression

   Yeast

   Mutation

   Mutated mCitrine for monomeric isoform (A206K L22…

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GTT-ir

    Plasmid

    #81112

    Purpose

    Gene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    tdiRFP
    

    Use

    Cre/Lox

    Tags

    tdiRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GTL-r

    Plasmid

    #81195

    Purpose

    Gene Tagging vector LEU/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCherry
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GAL-CDC12(E368Q)

    Plasmid

    #1164

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    CDC12 (CDC12 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    E368Q

    Promoter

    GAL

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPS1152

    Plasmid

    #1378

    Depositor

    Pamela Silver

    Article

    Lee et al Genes Dev 1996 May 15;10(10):1233-46.

    Insert

    NPL3 (NPL3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RMC

    Plasmid

    #1173

    Depositor

    Susan Lindquist

    Article

    Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

    Insert

    Rnq1-Sup35 fusion (SUP35, RNQ1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    *(see below). Contains Rnq aa's 153-393 and…

    Promoter

    Available since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHU

    Plasmid

    #64172

    Purpose

    harbours marker genes HIS3 and URA3

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG424GPD-ECFP-ccdB

    Plasmid

    #14296

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 416Gal-FUS-R514S-YFP

    Plasmid

    #29617

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R514S

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pSB819-PKR-hum-FFA

    Plasmid

    #20048

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

    Insert

    Protein kinase R (EIF2AK2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L394F, S492A

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pAG423GPD-Cerulean-ccdB

    Plasmid

    #14414

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 413-Met25 Kar2 TorsinADE E171Q GFP

    Plasmid

    #32128

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    deletion of E at 302 or 303, E171Q

    Promoter

    Available since

    Oct. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pYES2.1-ScDcr1deltaN

    Plasmid

    #32038

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    Drc1(111-610)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pTH340-SUP45(1-242)-GalBD

    Plasmid

    #29740

    Depositor

    Tobias von der Haar

    Insert

    SUP45 (SUP45 Budding Yeast)
    

    Use

    Tags

    Gal Activation domain and Myc

    Expression

    Yeast

    Mutation

    Codons 1-242 of the yeast SUP45 gene only

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNC955

    Plasmid

    #20405

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    CFP-reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=UbiY (see schematic) linker=deltak

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p413TEF-PYK2

    Plasmid

    #34606

    Depositor

    Markus Ralser

    Article

    Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

    Insert

    PYK2 (PYK2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLM

    Plasmid

    #64175

    Purpose

    harbours marker genes LEU2 and MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG304GAL-EYFP-ccdB

    Plasmid

    #14327

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAG304GPD-EYFP-ccdB

    Plasmid

    #14328

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAG305GPD-ccdB-ECFP

    Plasmid

    #14162

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSB819-PKR-gib

    Plasmid

    #20031

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

    Insert

    Protein kinase R
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

30. PP161

    Plasmid

    #78993

    Purpose

    Supplemental Figure 4. Promoter GAP3. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PP164

    Plasmid

    #78988

    Purpose

    Supplemental Figure 4. Promoter short ppTEF1. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PP149

    Plasmid

    #78989

    Purpose

    Supplemental Figure 4. Promoter WT GAP. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PP154

    Plasmid

    #78991

    Purpose

    Supplemental Figure 4. Promoter GAP1. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PP160

    Plasmid

    #78992

    Purpose

    Supplemental Figure 4. Promoter GAP2. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PP155

    Plasmid

    #78994

    Purpose

    Supplemental Figure 4. Promoter GAP4. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PP162

    Plasmid

    #78995

    Purpose

    Supplemental Figure 4. Promoter GAP5. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PP163

    Plasmid

    #78996

    Purpose

    Supplemental Figure 4. Promoter GAP6. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG416GPD-Cerulean-YPT6

    Plasmid

    #18845

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT6 (YPT6 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

39. pREP3xV5_ccdB

    Plasmid

    #79015

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Use

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p41Nat 1-F GW

    Plasmid

    #58546

    Purpose

    CEN vector with clonNat resistance marker for single Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge