8,350 results
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Plasmid#207033PurposeExpression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.DepositorInsertATG8
Tags3xHAExpressionYeastMutation∆GRPromoterpATG8Available SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Sap
Plasmid#206856PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-mRuby3-Pho8-406
Plasmid#207043PurposeExpression of mRuby3-Pho8 under CPU1 promoter.DepositorInsertPHO8
TagsmRuby3ExpressionYeastPromoterpCUP1Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXOOY
Plasmid#196449PurposeA dual vector for expression of membrane proteins in S. cerevisiae and X. laevisDepositorTypeEmpty backboneUseExpression in xenopus laevisExpressionYeastPromoteryeast CYC-GAL and T7 promoterAvailable SinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Emc1-GFP-APEX2-Ura
Plasmid#207074PurposeExpressing Emc1-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertEmc1
TagsGFP-APEX2ExpressionYeastPromoterpTPI1Available SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Cox4-GFP-APEX2-Ura
Plasmid#207075PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
TagsGFP-APEX2ExpressionYeastPromoterpTPI1Available SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
IAA17-4flag-HIS5
Plasmid#207015PurposeFor ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-HIS5
Plasmid#207014PurposePlasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-MET15
Plasmid#207013PurposePlasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-URA3
Plasmid#207012PurposePlasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-NLS-VSV
Plasmid#212746PurposeDoxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastPromoterTetAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-VSV
Plasmid#212752PurposeDoxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterTetAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-TET-NUbo-E3(63)-VSV
Plasmid#212753PurposeDoxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterTetAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-NLS-VSV
Plasmid#212756PurposeDoxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastPromoterTetAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-TET-NUbo-E3(48)-VSV
Plasmid#212744PurposeDoxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterTetAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-VSV
Plasmid#212743PurposeDoxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterTetAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD140
Plasmid#212927PurposeEncodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD113
Plasmid#212926PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRH3105
Plasmid#210164PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-P194Q-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::lys1-P194Q-GFP::tADH1 (LYS1 Budding Yeast)
ExpressionYeastMutationP194QAvailable SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His7-NUbo-GFP
Plasmid#212791PurposeCopper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeastDepositorInsertHis7-NUbo-GFP
UseIntegrative vectorExpressionYeastPromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo-GFP
Plasmid#212792PurposeCopper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeastDepositorInsertHis6-CUbo-GFP
UseIntegrative vectorExpressionYeastPromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R)-GFP
Plasmid#212793PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K48R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K63R)-GFP
Plasmid#212794PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K63R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K63R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP
Plasmid#212795PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R/K63R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K48R/K63R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-CUbo-AID*-GFP
Plasmid#212796PurposeCopper-inducible expression of the artificial test substrate His6-CUbo-AID*-GFP in budding yeastDepositorInsertCUbo-AID*-GFP
UseIntegrative vectorExpressionYeastPromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(63)-mCherry-VSV
Plasmid#212759PurposeConstitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and mCherry-VSVExpressionYeastPromoterADH1Available SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(63)-mCherry-VSV
Plasmid#212758PurposeConstitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and mCherry-VSVExpressionYeastPromoterADH1Available SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-CUP1-NUbo-E3(63) I227A-VSV
Plasmid#212757PurposeCopper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)DepositorInsertE3(63) I227A
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPib1(I227A)PromoterCUP1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(63)-NLS-VSV
Plasmid#212755PurposeConstitutive expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-CUP1-NUbo-E3(63)-VSV
Plasmid#212754PurposeCopper-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterCUP1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(63)-VSV
Plasmid#212751PurposeConstitutive expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(63)-VSV
Plasmid#212750PurposeConstitutive expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48)-mCherry-VSV
Plasmid#212749PurposeConstitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and mCherry-VSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-mCherry-VSV
Plasmid#212748PurposeConstitutive expression of NUbo-E3(48)-mCherry-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and mCherry-VSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48) aa24-150-VSV
Plasmid#212747PurposeConstitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)DepositorInsertE3(48) aa24-150
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationCue1 ΔU7BDPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-NLS-VSV
Plasmid#212745PurposeConstitutive expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48)-VSV
Plasmid#212742PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-VSV
Plasmid#212741PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV
Plasmid#212740PurposeConsitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-mCherry-VSV and mycExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV
Plasmid#212739PurposeConsitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-mCherry-VSV and mycExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1) C885A-CUbo-VSV
Plasmid#212738PurposeConsitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)DepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-NLS-VSV and mycExpressionYeastMutationHOIP(C885A)PromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV
Plasmid#212737PurposeConsitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-NLS-VSV and mycExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1)-CUbo-VSV
Plasmid#212736PurposeConsitutive expression of myc-E3(1)-CUbo-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-VSV and mycExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-myc-E3(1)-CUbo-VSV
Plasmid#212735PurposeConsitutive expression of myc-E3(1)-CUbo-VSV in budding yeastDepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-VSV and mycExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-CUbo-mCherry-VSV
Plasmid#212734PurposeConsitutive expression of CUbo-mCherry-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsmCherry-VSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-CUbo-VSV
Plasmid#212733PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only