8,173 results

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pFA6a-link-yoPA-mCherry-SpHis5

Plasmid

#44855

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-nLuc

Plasmid

#199756

Purpose

Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-nLuc

Expression

Yeast

Promoter

TetO7

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pH3FS-GAL1

Plasmid

#85782

Purpose

N-ICE plasmid pH3FS-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pOP462

Plasmid

#85935

Purpose

Encodes the Anti-Ebola 13C6 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

Depositor

Timothy Lu

Article

Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

Insert

Anti-Ebola 13C6 monoclonal antibody

Expression

Yeast

Promoter

AOX1

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pKT0139

Plasmid

#8731

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pDR FLIP43 GPS1-NR

Plasmid

#101085

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1-NR

Tags

6HIS and cMyc

Expression

Yeast

Mutation

AtGAI E51K E54R, AtGID1C S114A F115A

Promoter

pPMA1

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAG414GAL-ccdB-HA

Plasmid

#14239

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pPTK003-2-pENO1

Plasmid

#84962

Purpose

Type 2, pENO1, Enolase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Enolase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (2350 g to c) and BsmBI site re…

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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YIplac211-SYS1-msGFPx3

Plasmid

#177112

Purpose

Expresses Sys1-msGFP3 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SYS1 (SYS1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP4M

Plasmid

#104508

Purpose

Used to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SeGAL2 promoter-yEGFP

Expression

Yeast

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046348

Plasmid

#149108

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDDGFP-Leu2d-GgKDELR2

Plasmid

#123618

Purpose

Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDRFLIP42

Plasmid

#65518

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-Cit-attR1 and attR2-mCer-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP35

Plasmid

#65513

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-TFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP32

Plasmid

#65511

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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BS-Leu2

Plasmid

#69200

Purpose

complementation of yeast auxotrophic marker LEU2

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

LEU2

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFA CRU HISMX

Plasmid

#131164

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

CRU

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFA NuiHA-loxP-nat-CUP1p

Plasmid

#131170

Purpose

PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-170nΔ13V

Plasmid

#27999

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-170nΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

WT binding protein (no F16A)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pBS4

Plasmid

#83793

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Tags

CFP

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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