8,060 results

1. pL-eCFP

   Plasmid

   #176556

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of leucine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU-eCFP

   Plasmid

   #176557

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHES609 [prNOp7-PBAC]

   Plasmid

   #103017

   Purpose

   A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

   Depositor

   Hana El-Samad

   Article

   Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

   Insert

   bPAC
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDR-GW FLIPglu-30μΔ13V

   Plasmid

   #27998

   Depositor

   Wolf Frommer

   Article

   Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

   Insert

   FLIPglu-30μΔ13V
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Yeast

   Mutation

   D154A in mglB

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pAW

   Plasmid

   #64745

   Purpose

   Yeast expression plasmid: ACC1

   Depositor

   Jens Nielsen

   Article

   Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

   Insert

   ACC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETCON-DnvLB16

   Plasmid

   #45124

   Depositor

   David Baker

   Article

   Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

   Insert

   DnvLB16
   

   Use

   Tags

   Aga2p for yeast surface display and c-myc epitope…

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pV1329

   Plasmid

   #111438

   Purpose

   Solo vector pV1326 +sgCgADE2

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgCgADE2
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG413GPD-EGFP-ccdB

   Plasmid

   #14310

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG424GAL-EGFP-ccdB

   Plasmid

   #14319

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pR5-28

    Plasmid

    #22289

    Depositor

    Louise Prakash

    Article

    Pages et al Genetics. 2008 Sep . 180(1):73-82.

    Insert

    RAD5 (RAD5 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pCfB2226

    Plasmid

    #67533

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDH3

    Plasmid

    #83789

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    F64L, Y66W, N146I, M153T, V163A, N164H mutations …

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-link-yomKO2-CaURA3

    Plasmid

    #44881

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKO2 with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDRF1-GW mScarletI-T2A-mTurquoise2

    Plasmid

    #118430

    Purpose

    Produces equimolar expression of mScarletI and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    mScarletI-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDN-G1Gh

    Plasmid

    #44507

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pROS12

    Plasmid

    #107926

    Purpose

    hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RPL3-GFP (96)

    Plasmid

    #24040

    Depositor

    Michael Rout

    Insert

    RPL3 (RPL3 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

19. YIplac211-APL5-mCherry2Bx6

    Plasmid

    #105253

    Purpose

    Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Apl5 (APL5 Budding Yeast)
    

    Use

    Tags

    mCherry2Bx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-link-yECerulean-CaUra3

    Plasmid

    #44648

    Depositor

    Kurt Thorn

    Insert

    yECerulean
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge