8,432 results
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Plasmid#84962PurposeType 2, pENO1, Enolase 1 promoterDepositorInsertEnolase 1 promoter
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (2350 g to c) and BsmBI site re…PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK016-3-RFP_Intra
Plasmid#84975PurposeType 3, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PATagRFP-KanMX6
Plasmid#87027PurposeTag S. pombe genes in C terminal with PATagRFPDepositorInsertPATagRFP
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
Tagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PP75
Plasmid#78945PurposeSupplemental Figure 3. Integration Site 2.DepositorInsertIntegration Site 2
ExpressionYeastAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3F
Plasmid#85773PurposeN-ICE plasmid pH3FDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-PYK1
Plasmid#85777PurposeN-ICE plasmid pK3FS-PYK1DepositorInsertloxP-KanMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
ExpressionYeastPromoterpFUS1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-FKBP(M)x4
Plasmid#85003PurposeExpresses EGFP-FKBP(M)x4 in yeastDepositorInsertEGFP-FKBP(M)x4
ExpressionYeastAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(L)
Plasmid#85001PurposeExpresses E2-Crimson-FKBP(L)DepositorInsertE2-Crimson-FKBP(L)
ExpressionYeastAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3049(gRNA XII-4)
Plasmid#73291PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3053(gRNA X-2, XI-5, XII-4)
Plasmid#73295PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCfB3043(gRNA XI-1)
Plasmid#73285PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNXRVj-HACre
Plasmid#78407Purposeconstitutive Cre expression vectorDepositorAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FTKanAX
Plasmid#73900Purposefission yeast drug counter-selectable selection markerDepositorInsertTK-kan
ExpressionYeastAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTH-g
Plasmid#81104PurposeGene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GTT-r
Plasmid#81111PurposeGene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEW8
Plasmid#84238PurposePlasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiaeDepositorInsertHA-mRuby-P2A-eGFP
TagsHA-mRuby and eGFPExpressionYeastPromoterGPDAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS7
Plasmid#83774PurposeUseful for tagging yeast proteins with C-terminal Venus tag; G418 selectionDepositorPublicationInsertVenus
TagsVenusExpressionYeastMutationF46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
ExpressionYeastPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by