8,086 results
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Plasmid#45124DepositorInsertDnvLB16
UseTagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastMutationPromoterGALAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec7-mScarlet2Ix6
Plasmid#140151PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6DepositorInsertSEC7
UseTagsExpressionYeastMutationPromoterAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYC54
Plasmid#63909PurposeRecipient integrative vector to construct YFP tagged fusionsDepositorTypeEmpty backboneUseTagsYFPExpressionYeastMutationPromoterAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ1A
Plasmid#197381PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-1 σ1A
UseTagsGAL4-DNA binding domain fragment, HA tag, and SV4…ExpressionYeastMutationContains an extra 42 nucleotides encoding 14 resi…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHES820
Plasmid#89190PurposeExpresses YFP under control of GAL1 promoter.DepositorInsertpGAL1-YFP
UseTagsExpressionYeastMutationPromoterGAL1Available SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL5-mCherry2Bx6
Plasmid#105253PurposePlasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-KHC-BZ
Plasmid#160427PurposeExpression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV1464
Plasmid#111440PurposeN. castellii Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK3F-GAL1
Plasmid#85772PurposeN-ICE plasmid pK3F-GAL1DepositorInsertloxP-KanMX-loxP-GAL1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB6677
Plasmid#106155PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2226
Plasmid#67533PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-CYC1
Plasmid#85779PurposeN-ICE plasmid pK3FS-CYC1DepositorInsertloxP-KanMX-loxP-CYC1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pJY4092
Plasmid#113334PurposeVector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoterDepositorInsertcodon-optimized Hermes transposase
UseTagsExpressionYeastMutationPromoterAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterADH1Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pROS15
Plasmid#107929Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH-eCFP
Plasmid#176555PurposeExpression of eCFP for auxotrophic selection in the absence of histidineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-eCFP
Plasmid#176556PurposeExpression of eCFP for auxotrophic selection in the absence of leucineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only