8,060 results
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Plasmid#17421DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-GGA2-HaloTagx2
Plasmid#140150PurposePlasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2DepositorInsertGGA2
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsCERK
Plasmid#118600PurposeExpresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphateDepositorInsertHomo sapiens ceramide kinase
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceFeb. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Tt
Plasmid#44509DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Desmin/pAS2-1
Plasmid#97407PurposeDesmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertDesmin (DES Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-link-mKO1-SpHis5
Plasmid#44642PurposeYeast tagging vector to create gene fusion with mKO1 with SpHis5 selectionDepositorInsertmKO1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-DsRed
Plasmid#14358DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB-EYFP
Plasmid#14205DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Destination Vector
Plasmid#125754PurposeDestination vector for assembling gRNA fragments in the second round of assembly.DepositorInsertpTDH3-GFPdropout-tTDH1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-ECFP
Plasmid#14180DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pZHY500
Plasmid#36186DepositorInsertFokI homodimeric Nuclease domain
UseTagsACV5ExpressionYeastMutationdelta152,+63 truncation to TAL backbonePromoterTEVAvailable sinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
D5 gene in PETCON vector
Plasmid#87626Purposeexpresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast displayDepositorInsertDesigned allosteric Zn2+ and fluorescein binding antibody scFv D5
UseTags3x(G4S) Linker and cMyc tag on backboneExpressionYeastMutationPromoterAvailable sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-P3nmt1-3FLAG
Plasmid#19348DepositorInserthphMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FTKanAX
Plasmid#73900Purposefission yeast drug counter-selectable selection markerDepositorInsertTK-kan
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046418
Plasmid#149029PurposeYeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-GBP-RFP-hphMX6
Plasmid#105161PurposeYeast gene targetingDepositorInsertGBP-RFP-hphMX6
UseTagsGBP-RFPExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-RFP
Plasmid#166841PurposeExpresses Vac8-RFP in yeastsDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsRFPExpressionYeastMutationPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only