8,133 results

1. pCfB2989

   Plasmid

   #63636

   Purpose

   pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. RPL25NLS-GFP (101)

   Plasmid

   #24038

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Only bp 1-135 (aa 1-45) of RPL25

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pXP822

   Plasmid

   #46064

   Depositor

   Nancy DaSilva

   Article

   Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMEG_BB3_YL001

   Plasmid

   #117825

   Purpose

   Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Insert

   YlpTEF_YlGUT1_YlCyc1TT
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-yoEmerald-Kan

   Plasmid

   #44946

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEmerald
   

   Expression

   Yeast

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4046457

   Plasmid

   #145424

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGBW-m4046390

   Plasmid

   #148996

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pFS485

   Plasmid

   #89348

   Purpose

   Leu1 integrating plasmid with cdc25 genomic fragment

   Depositor

   Nick Rhind

   Article

   Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

   Insert

   cdc25 (cdc25 Fission Yeast)
   

   Expression

   Yeast

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046332

   Plasmid

   #145413

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. GTH-y

    Plasmid

    #81105

    Purpose

    Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCitrine
    

    Use

    Cre/Lox

    Tags

    mCitrine

    Expression

    Yeast

    Mutation

    Mutated mCitrine for monomeric isoform (A206K L22…

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB8383

    Plasmid

    #126917

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    tHMG1
    

    Expression

    Yeast

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. YIplac211-iGFP-YPT31

    Plasmid

    #105263

    Purpose

    Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Ypt31 (YPT31 )
    

    Tags

    iGFP

    Expression

    Yeast

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. EZ-L767

    Plasmid

    #131773

    Purpose

    PADH1_sFUS_Fusion Red_PixD_TACT1

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_sFUS_Fusion Red_PixD_TACT1
    

    Use

    Integration into δ-sites

    Expression

    Yeast

    Promoter

    PADH1

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. BiFC3

    Plasmid

    #190048

    Purpose

    BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (ATG-aa173-238)
    

    Expression

    Yeast

    Promoter

    Met3

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. BiFC4

    Plasmid

    #190049

    Purpose

    BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (ATG-aa173-238)
    

    Expression

    Yeast

    Promoter

    Met3

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. BiFC2

    Plasmid

    #190047

    Purpose

    BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (aa1-172 - TAA)
    

    Expression

    Yeast

    Promoter

    Met3

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. BiFC1

    Plasmid

    #190046

    Purpose

    BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (aa1-172)
    

    Expression

    Yeast

    Promoter

    Met3

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFS177

    Plasmid

    #12380

    Depositor

    Nick Rhind

    Article

    Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

    Insert

    human equilibrating nucleoside transporter 1 (SLC29A1 Human)
    

    Expression

    Yeast

    Mutation

    N81S and M84V

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIplac211-CHC1-msGFPx3

    Plasmid

    #105256

    Purpose

    Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Chc1 portion (CHC1 Budding Yeast)
    

    Tags

    msGFPx3

    Expression

    Yeast

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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