8,266 results
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Plasmid#149059PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW Electra1-T2A-mCherry
Plasmid#205498PurposeExpression of Electra1-T2A-mCherry in yeastDepositorInsertElectra1-T2A-mCherry
ExpressionYeastAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJZC593
Plasmid#62319PurposesgRNA with MS2-PP7 for yeast cellsDepositorInsertsgRNA + MS2-PP7 RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS7
Plasmid#83774PurposeUseful for tagging yeast proteins with C-terminal Venus tag; G418 selectionDepositorInsertVenus
TagsVenusExpressionYeastMutationF46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …Available SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF -LEU- 4xFLAG
Plasmid#182527PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsert7 Copies of TetO Box
Tags4XFLAGExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179035PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulumDepositorInsertRPL18Bp-Neon(gfp)-Link-SCS2tmh
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Spt2-GFP
Plasmid#115572PurposeExpresses yeast Spt2-GFP fusion proteinDepositorInsertSPT2
TagsGFPExpressionBacterial and YeastAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LD220
Plasmid#69188PurposeIntegration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg20-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB6682
Plasmid#106152PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB1751
Plasmid#174013PurposepDUAL plasmid expressing GFP from 41nmt1 promoterDepositorInsertGFP
TagsGFPExpressionYeastPromoter41nmt1 and 41nmt1 promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW eGFP-T2A-mCherry
Plasmid#118422PurposeProduces equimolar expression of eGFP and mCherryDepositorInserteGFP-T2A-mCherry
ExpressionYeastAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK084
Plasmid#171356PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Met] A.niger Pol-III terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB23-L-beta-gal
Plasmid#8820DepositorInsertbeta-gal
TagsUbiquitinExpressionYeastMutationL (leucine) at amino terminus of beta-galAvailable SinceDec. 28, 2005AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS8-iGFPx6
Plasmid#105271PurposePlasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-EYFP
Plasmid#14220DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SNF7
Plasmid#51574PurposeConstitutively express endosome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsSNF7 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFTK039
Plasmid#171311PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertpyrG AO090011000868 Orotidine 5'-phosphate decarboxylase, selection marker
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1
Plasmid#182512PurposeC-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.DepositorInsertCL1 Degron
TagsGFPExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS2
Plasmid#12389DepositorInsertgreen fluorescent protein
ExpressionYeastAvailable SinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only