8,043 results

1. pCEV-G2-Km ymTurquoise2

   Plasmid

   #193958

   Purpose

   TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGBT9-GGA2-GAE

   Plasmid

   #199175

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA2-GAE domain (GGA2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Contains Lys 545 Gln substitution

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBT9-γ1 ear

   Plasmid

   #199177

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   AP-1 γ1 ear domain (Ap1g1 Mouse)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBT9-GGA3-GAE

   Plasmid

   #199176

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA3-GAE domain (GGA3 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBT9-γ2 ear

   Plasmid

   #199178

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   AP-1 γ2 ear domain (AP1G2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBT9-GGA1-GAE

   Plasmid

   #199174

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA1-GAE domain (GGA1 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBT9-ATG9A-N

   Plasmid

   #198530

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

   Insert

   ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBT9-FTS

   Plasmid

   #198526

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FTS (AKTIP Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBT9-FHIP-L

   Plasmid

   #198528

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FHIP-L (FHIP1A Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBT9-Hook1

    Plasmid

    #198523

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook1 (HOOK1 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent substitution in codon 620 to eliminate int…

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGADT7-δ

    Plasmid

    #197259

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-3 δ (AP3D1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Val 549 Ile substitution; silent substitutions in…

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGAD424-Rabex-5

    Plasmid

    #197043

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    Rabex-5 (RABGEF1 Bovine)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pROS_phleo-Tps1/Gsy2

    Plasmid

    #196612

    Purpose

    Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGADT7-AP-4 β4

    Plasmid

    #198327

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-4 β4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Contains L480S substitution

    Promoter

    ADH1

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAD424-AP-4 σ4

    Plasmid

    #198328

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-4 σ4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGADT7-AP-2 σ2

    Plasmid

    #198345

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-2 σ2
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    R42G substitution, and silent substitutions in co…

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGADT7-AP-1 σ1B

    Plasmid

    #198335

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGADT7-AP-1 σ1A

    Plasmid

    #198334

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGADT7-AP-1 σ1C

    Plasmid

    #198336

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1C
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    S148C substitution

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pACT2-AP-3 σ3A

    Plasmid

    #198347

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-3 σ3A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pACT2-AP-3 σ3B

    Plasmid

    #198348

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-3 σ3B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pACT2-μ1B (137-423)

    Plasmid

    #198172

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-1 μ1B (137-423)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOAD eIF4G1 (1-341)

    Plasmid

    #196833

    Purpose

    Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

    Depositor

    Stan Fields

    Article

    Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

    Insert

    eIF4G1 (TIF4631 Budding Yeast)
    

    Use

    Tags

    Gal4

    Expression

    Yeast

    Mutation

    amino acids 1-341

    Promoter

    ADH

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pACT2-μ1A

    Plasmid

    #198168

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-1 μ1A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pACT2-μ2

    Plasmid

    #198173

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-2 μ2
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGBT9-AAGAB

    Plasmid

    #198332

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AAGAB (AAGAB Human)
    

    Use

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOBD2 Pab1 WT (123-204)

    Plasmid

    #196834

    Purpose

    Expresses Pab1 RRM2 fragment fused to GAL4 DNA binding domain

    Depositor

    Stan Fields

    Article

    Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

    Insert

    Pab1 (PAB1 Budding Yeast)
    

    Use

    Tags

    Gal4

    Expression

    Yeast

    Mutation

    123-204 amino acids

    Promoter

    ADH

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOBD2 Pab1 E181R (123-204)

    Plasmid

    #196835

    Purpose

    Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain

    Depositor

    Stan Fields

    Article

    Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

    Insert

    Pab1 (PAB1 Budding Yeast)
    

    Use

    Tags

    Gal4

    Expression

    Yeast

    Mutation

    123-204 amino acids & E181R

    Promoter

    ADH

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pROS13-Tps2/Gsy1

    Plasmid

    #196613

    Purpose

    Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    S000001911 (TPS2, GSY1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGADT7-γ1

    Plasmid

    #197078

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-1 γ1 (Ap1g1 Mouse)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGADT7-ε

    Plasmid

    #197261

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-4 ε (AP4E1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitutions in codons 905, 1129 and 1137

    Promoter

    ADH1

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGBT9-tepsin

    Plasmid

    #196647

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    tepsin (TEPSIN Human)
    

    Use

    Yeast two-hybrid vector

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent substitution in codon 199 to eliminate int…

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GA46

    Plasmid

    #196614

    Purpose

    Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUGP1.1

    Plasmid

    #196615

    Purpose

    Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. UG75-PAH1-3HA

    Plasmid

    #184761

    Purpose

    PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    PAH1 (PAH1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    PAH1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. UG75-CDS1-3HA

    Plasmid

    #184760

    Purpose

    CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    CDS1 (CDS1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    CDS1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. UG75-DGK1-3HA

    Plasmid

    #184759

    Purpose

    DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    DGK1 (DGK1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    DGK1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAS-mak10 gRNA-MAK10Δ

    Plasmid

    #160384

    Purpose

    For CRISPR-mediated deletin og MAK10 in yeast

    Depositor

    John McCusker

    Insert

    MAK10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAS-mak10 gRNA

    Plasmid

    #160385

    Purpose

    Expresses guide RNA for targeting MAK10 in yeast

    Depositor

    John McCusker

    Insert

    mak10 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RNA pol III promoter (tRNA-Tyr)

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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