7,935 results

1. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE URA3

   Plasmid

   #122882

   Purpose

   URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pATP416-­ptetO7-­pphlO6-­crtYBI

   Plasmid

   #165976

   Purpose

   Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Inserts

   Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
   Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
   BTS1 (BTS1 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   C1281A, G1698A and C66A, C1359T

   Promoter

   Synthetic promoter (pphlO6), Synthetic promoter (…

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pI6-MTU-DO-URA

   Plasmid

   #160030

   Purpose

   Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

   Plasmid

   #173456

   Purpose

   Contains mScarletH-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mScarlet-H-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-yomEos2-Kan

   Plasmid

   #44944

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomEos2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG426-GAL-TAF15

   Plasmid

   #181713

   Purpose

   Galactose inducible expression of TAF15

   Depositor

   Alejandro Chavez

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416-GAL-hnRNPA1

   Plasmid

   #181710

   Purpose

   Galactose inducible expression of hnRNPA1

   Depositor

   Alejandro Chavez

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIplac211-VPS8-HaloTagx2

   Plasmid

   #177113

   Purpose

   Expresses Vps8-HaloTagx2 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   VPS8 (VPS8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJW1661

    Plasmid

    #112035

    Purpose

    OsTIR1 expression cassette marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    TIR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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