7,943 results

1. pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

   Plasmid

   #68276

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7DS NLS(4E) mCherry
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   RPS2

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCfB2336

   Plasmid

   #67541

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK275

   Plasmid

   #73363

   Purpose

   Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)

   Depositor

   T. J. Kappock

   Insert

   delta-8 sphingolipid desaturase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   missing residues 1-6

   Promoter

   GAL1

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK138

   Plasmid

   #73314

   Purpose

   Pas2p peroxisomal membrane targeting sequence

   Depositor

   T. J. Kappock

   Insert

   Pas2p (peroxisomal membrane targeting region)
   

   Use

   Tags

   His6 and c-myc

   Expression

   Yeast

   Mutation

   encodes residues 1-42

   Promoter

   AOX1

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK141

   Plasmid

   #73316

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag

   Depositor

   T. J. Kappock

   Insert

   delta-12 fatty acid acetylenase
   

   Use

   Tags

   His6, Pas2p signal sequence, and c-myc

   Expression

   Yeast

   Mutation

   synthetic gene

   Promoter

   AOX1

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK137

   Plasmid

   #73313

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag

   Depositor

   T. J. Kappock

   Insert

   delta-12 fatty acid acetylenase
   

   Use

   Tags

   His6 and c-myc

   Expression

   Yeast

   Mutation

   synthetic gene

   Promoter

   5'-AOX1

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTD125 GFP:POP1

   Plasmid

   #71387

   Purpose

   localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

   Depositor

   Mark Schmitt

   Article

   Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

   Insert

   POP1 (POP1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   ACT1

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pUG35-YDL183c-GFP

   Plasmid

   #70161

   Purpose

   yeast expression of YDL183c with GFP fusion

   Depositor

   Karin Nowikovsky

   Article

   Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

   Insert

   YDL183c
   

   Use

   Tags

   yEGFP

   Expression

   Yeast

   Mutation

   Promoter

   MET17

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

9. YCp33-YDL183c-HA

   Plasmid

   #70164

   Purpose

   yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end

   Depositor

   Karin Nowikovsky

   Article

   Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

   Insert

   YDL183c
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   Promoter

   YDL183c (217 nucleotides upstream of the ATG)

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pJK349

    Plasmid

    #71566

    Purpose

    Produces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid acetylenase
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDS2-aurZ

    Plasmid

    #70151

    Purpose

    Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    aurZ
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMES140

    Plasmid

    #71849

    Purpose

    Complementation of NME1 gene disruption or mutation in yeast

    Depositor

    Mark Schmitt, David Clayton

    Article

    Schmitt et al Mol Cell Biol. 1993 Dec;13(12):7935-41.

    Insert

    NME1 (NME1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    NME1

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK274

    Plasmid

    #71564

    Purpose

    Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid desaturase (form 3)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1 promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK272

    Plasmid

    #71562

    Purpose

    Produces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid acetylenase
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDS3-aurJ

    Plasmid

    #70152

    Purpose

    Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    aurJ
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD1

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDS3-X4

    Plasmid

    #70140

    Purpose

    Yeast chr. integration vector with DS3 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    DS3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AgTEF1

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS425-M25-Afg1

    Plasmid

    #61720

    Purpose

    yeast Afg1 AAA protein in yeast expression vector

    Depositor

    Oleh Khalimonchuk

    Article

    Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

    Insert

    Afg1 (AFG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pRS415-Cox15-13xMyc

    Plasmid

    #61721

    Purpose

    yeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vector

    Depositor

    Oleh Khalimonchuk

    Article

    Swenson et al J Biol Chem. 2016 Mar 3. pii: jbc.M115.7

    Insert

    Cox15 (COX15 Budding Yeast)
    

    Use

    Tags

    13xMyc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCfB2229

    Plasmid

    #67537

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LD123

    Plasmid

    #69173

    Purpose

    Integration of GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg23-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LD114

    Plasmid

    #69167

    Purpose

    Integration of GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg14-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LD105

    Plasmid

    #69162

    Purpose

    Integration of GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg5-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BS-Trp1

    Plasmid

    #69196

    Purpose

    complementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    TRP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCyc-Bem1

    Plasmid

    #66573

    Purpose

    Express an additional copy of Bem1, integrated at TRP locus

    Depositor

    Orion Weiner

    Article

    Jost et al ACS Synth Biol. 2015 Jun 2.

    Insert

    Bem1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F99I*(see below)

    Promoter

    pCyc1

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LD131

    Plasmid

    #69177

    Purpose

    Integration of GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg31-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LD129

    Plasmid

    #69176

    Purpose

    Integration of GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg29-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LD124

    Plasmid

    #69174

    Purpose

    Integration of GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg24-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LD120

    Plasmid

    #69171

    Purpose

    Integration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg20-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pED55 pRS306 pRPS2-SteND-NLS-mCherry

    Plasmid

    #68277

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7ND NLS mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

    Plasmid

    #68275

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS(4A) mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCyc-Cdc24

    Plasmid

    #66574

    Purpose

    Express an additional copy of Cdc24, integrated at TRP locus

    Depositor

    Orion Weiner

    Article

    Jost et al ACS Synth Biol. 2015 Jun 2.

    Insert

    Cdc24
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pCyc1

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRPR1_g2gRNA_RPR1t

    Plasmid

    #64388

    Purpose

    encodes g2 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    g2 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pRPR1

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pRS424-TUB2 - human TUBB3 Cterminal tail delta K

    Plasmid

    #60404

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pRS424-TUB2 - human TUBB1 Cterminal tail

    Plasmid

    #60400

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pRS424-TUB2 - human TUBB3 Cterminal tail

    Plasmid

    #60397

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C127S; TUB2 amino acids 429 - end, replaced with …

    Promoter

    GAL

    Available since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pRPR1_c2gRNA_RPR1t

    Plasmid

    #64380

    Purpose

    encodes c2 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c2 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pRPR1_g1gRNA_RPR1t

    Plasmid

    #64387

    Purpose

    encodes g1 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    g1 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pRPR1_c7gRNA_RPR1t

    Plasmid

    #64385

    Purpose

    encodes c7 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c7 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pRPR1_c1gRNA_RPR1t

    Plasmid

    #64379

    Purpose

    encodes c1 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c1 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pRPR1_c5gRNA_RPR1t

    Plasmid

    #64383

    Purpose

    encodes c5 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c5 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

41. Gal4pBS13-Pleum

    Plasmid

    #60332

    Purpose

    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pJW1509

    Plasmid

    #62363

    Purpose

    pFA6a BirA-heh2 linker-SSH1::HIS5

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    BirA-heh2-Ssh1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGAD-hPLEKHM1

    Plasmid

    #64145

    Purpose

    For yeast two hybrid

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Use

    Tags

    GAL4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

44. pGAD-hRubicon

    Plasmid

    #64144

    Purpose

    For yeast two hybrid

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    GAL4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pNB786

    Plasmid

    #60151

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNB762

    Plasmid

    #60150

    Purpose

    Firefly luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pNB767

    Plasmid

    #60159

    Purpose

    Firefly luciferase (Promega) driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pNB682

    Plasmid

    #60143

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. pJW3.31

    Plasmid

    #62865

    Purpose

    Yeast two-hybrid bridge vector expressing PAA-1

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    PAA-1/Y3H
    

    Use

    Two-hybrid bridge vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRevLCav2deltaC

    Plasmid

    #60809

    Purpose

    to make truncation of yeast Rev3

    Depositor

    Youri Pavlov, Tahir Tahirov

    Article

    Siebler et al DNA Repair (Amst). 2014 May 9. pii: S156

    Insert

    REV3 (REV3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    rev3ΔC encodes for Rev3 lacking the C-terminus (a…

    Promoter

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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