8,265 results
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Plasmid#78964PurposePromoter: scTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP277
Plasmid#78967PurposePromoter: scTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP190
Plasmid#78969PurposePromoter: ppTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP276
Plasmid#78970PurposePromoter: GAP6, minimal Promoter: GCW14. Figure 4. Expresses GFP.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP297
Plasmid#78937PurposeExpresses GFP. Figure 3. Strain 3.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP298
Plasmid#78938PurposeExpresses GFP. Figure 3. Strain 4.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP299
Plasmid#78939PurposeExpresses GFP. Figure 3. Strain 6.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP300
Plasmid#78940PurposeExpresses GFP. Figure 3. Strain 7.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP228
Plasmid#78942PurposeExpresses GFP. Figures 3 and 4. Strain 8. Promoter: ppTEF1, minimal Promoter: mCYC.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP77
Plasmid#78947PurposeSupplemental Figure 3. Integration Site 4.DepositorInsertIntegration Site 4
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP79
Plasmid#78949PurposeSupplemental Figure 3. Integration Site 6.DepositorInsertIntegration Site 6
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP69
Plasmid#78950PurposeSupplemental Figure 3. Integration Site 7.DepositorInsertIntegration Site 7
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP295
Plasmid#78935PurposeExpresses GFP. Figure 3. Strain 1.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP296
Plasmid#78936PurposeExpresses GFP. Figure 3. Strain 2.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWHI2-HYG
Plasmid#80589PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorAvailable SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pcr1P-natMX6-pcr2P
Plasmid#80620Purposeto delete part of the mat1-P locus and fix the mating type in natural S. pombe isolatesDepositorInsertsPCR1 from mat1-P
PCR2 from mat1-P
ExpressionYeastAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pcr1M-natMX6-pcr2M
Plasmid#80621Purposeto delete part of the mat1-M locus and fix the mating type in natural S. pombe isolatesDepositorInsertsPCR1 from mat1-M
PCR2 from mat1-M
ExpressionYeastAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP81xV5_ccdB
Plasmid#79019PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selctionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP41xV5_ccdB
Plasmid#79017PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP42xN_ccdB
Plasmid#79024PurposeGateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP82xV5_ccdB
Plasmid#79020PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP42xV5_ccdB
Plasmid#79018PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA186
Plasmid#74250PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).DepositorInsertInducible peptide of the dPSTR
ExpressionBacterial and YeastPromoterpGPD1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA171
Plasmid#74251PurposeIntermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
TagsVenusExpressionBacterial and YeastPromoterpSTL1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA199
Plasmid#74275PurposepSTL1-dPSTRy(4,3)DepositorInsertpSTL1-dPSTRy(4,3)
ExpressionBacterial and YeastAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK139
Plasmid#73315PurposePas2p peroxisomal membrane biogenesis proteinDepositorInsertPas2p peroxisomal membrane targeting protein
TagsHis6 and c-mycExpressionYeastMutationEncodes a glycine-204 to serine mutationPromoterAOX1Available SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
TagsmCherryExpressionYeastPromoterRPS2Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2336
Plasmid#67541PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK275
Plasmid#73363PurposeEncodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)DepositorInsertdelta-8 sphingolipid desaturase
ExpressionYeastMutationmissing residues 1-6PromoterGAL1Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
TagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK141
Plasmid#73316PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
TagsHis6, Pas2p signal sequence, and c-mycExpressionYeastMutationsynthetic genePromoterAOX1Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK137
Plasmid#73313PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
TagsHis6 and c-mycExpressionYeastMutationsynthetic genePromoter5'-AOX1Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD125 GFP:POP1
Plasmid#71387Purposelocalization of yeast RNase POP1 (RNase MRP binding protein) in yeastDepositorAvailable SinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUG35-YDL183c-GFP
Plasmid#70161Purposeyeast expression of YDL183c with GFP fusionDepositorInsertYDL183c
TagsyEGFPExpressionYeastPromoterMET17Available SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
YCp33-YDL183c-HA
Plasmid#70164Purposeyeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal endDepositorInsertYDL183c
Tags3xHAExpressionYeastPromoterYDL183c (217 nucleotides upstream of the ATG)Available SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK349
Plasmid#71566PurposeProduces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
ExpressionBacterial and YeastPromoterGAL1Available SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS2-aurZ
Plasmid#70151PurposeFusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertaurZ
ExpressionYeastPromoterTefAvailable SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMES140
Plasmid#71849PurposeComplementation of NME1 gene disruption or mutation in yeastDepositorAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK274
Plasmid#71564PurposeProduces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeastDepositorInsertdelta12 fatty acid desaturase (form 3)
ExpressionBacterial and YeastPromoterGAL1 promoterAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK272
Plasmid#71562PurposeProduces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
ExpressionBacterial and YeastPromoterGAL1Available SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-aurJ
Plasmid#70152PurposeFusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection markerDepositorInsertaurJ
ExpressionYeastPromoterGPD1Available SinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS3-X4
Plasmid#70140PurposeYeast chr. integration vector with DS3 selection markerDepositorInsertDS3
ExpressionYeastPromoterAgTEF1Available SinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS425-M25-Afg1
Plasmid#61720Purposeyeast Afg1 AAA protein in yeast expression vectorDepositorInsertAfg1 (AFG1 Budding Yeast)
ExpressionYeastAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS415-Cox15-13xMyc
Plasmid#61721Purposeyeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vectorDepositorAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2229
Plasmid#67537PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD123
Plasmid#69173PurposeIntegration of GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg23-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD114
Plasmid#69167PurposeIntegration of GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg14-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD105
Plasmid#69162PurposeIntegration of GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg5-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
BS-Trp1
Plasmid#69196Purposecomplementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.DepositorInsertTRP1
ExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only