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Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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PP75
Plasmid#78945PurposeSupplemental Figure 3. Integration Site 2.DepositorInsertIntegration Site 2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046319
Plasmid#153866PurposeYeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
LD116
Plasmid#69168PurposeIntegration of GFP tag at Atg16 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg16-GFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046292
Plasmid#149062PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS313-Sad1-Wt
Plasmid#90308Purposeyeast expressionDepositorInsertSAD1
UseTagsno tagExpressionYeastMutationPromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to depositedā¦PromoterGAL1 and GAL10Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046249
Plasmid#148991PurposeYeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ymScarletI-ymNeongreen pDRF1-GW
Plasmid#168067PurposeFRET fusion between ymScarletI and ymNeongreen for use in yeastDepositorInsertymScarletI-ymNeongreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
RPL25NLS-GFP-PRA (102)
Plasmid#24041DepositorInsertRPL25 (RPL25 Budding Yeast)
UseTagsEGFP and PRAExpressionYeastMutationOnly bp 1-135 (aa 1-45) of RPL25PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
721-2µ-staHIS3
Plasmid#40611DepositorInsertHIS3 (HIS3 Budding Yeast)
UseTagsHAExpressionYeastMutationPromoterTDH3Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGY466
Plasmid#166661PurposeFor light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeastDepositorInsertLiCre
UseCre/LoxTagsExpressionYeastMutationCre: E340A, D341APromoterMET17Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046230
Plasmid#145454PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL4AAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SNX4-iGFPx3
Plasmid#105269PurposePlasmid for pop-in/pop-out replacement of Snx4 with Snx4-iGFPx3.DepositorInsertSnx4 portion (SNX4 Budding Yeast)
UseTagsiGFPx3ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG424-ccdB
Plasmid#70124PurposeYeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterNoneAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRG236
Plasmid#64534PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTIN-ura4
Plasmid#161808PurposeA ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombeDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPho1Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
EZ-L498
Plasmid#131771PurposePPGK1_FUSN_Citrine _PixE_TCYC1DepositorInsertPPGK1_FUSN_Citrine _PixE_TCYC1
UseIntegration into his3 locusTagsExpressionYeastMutationPromoterPGKAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046658
Plasmid#149226PurposeYeast Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits