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GAL-CDC12(K351N)

Plasmid

#1166

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Expression

Yeast

Mutation

K351N

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(E368Q)

Plasmid

#1167

Depositor

Susan Lindquist

Publication

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

E368Q

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Available since

Sept. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Available since

Sept. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Available since

Sept. 18, 2026

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-URA3

Plasmid

#122911

Purpose

LYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

July 31, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pcdc2short-MCP-NLS-tdSG

Plasmid

#255013

Purpose

expression of MCP-NLS-tandemStayGold from medium-strength P.cdc2 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.cdc2(355-1,AfeImut)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Mutation

1 nucleotide removed in cdc2 promoter to abolish …

Promoter

Spom\P.cdc2

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Plon1-MCP-NLS-tdSG

Plasmid

#255010

Purpose

expression of MCP-NLS-tandemStayGold from weak P.lon1 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.lon1(451-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.lon1

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-MCP-NLS-tdSG

Plasmid

#255011

Purpose

expression of MCP-NLS-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Ppak1-MCP-NLS-tdSG

Plasmid

#255012

Purpose

expression of MCP-NLS-tandemStayGold from weak P.pak1 (P.shk1) promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.pak1(630-1)-MCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.pak1

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-MCP-2xNLS-NES-tdSG

Plasmid

#255014

Purpose

expression of MCP-2xNLS-NES-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-MCP-2xNLS-NES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-MCP-2xNLS-2xNES-tdSG

Plasmid

#255015

Purpose

expression of MCP-2xNLS-2xNES-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-MCP-2xNLS-2xNES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pcdc2short-MCP-2xNLS-NES-tdSG

Plasmid

#255016

Purpose

expression of MCP-2xNLS-NES-tandemStayGold from medium-strength P.cdc2 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.cdc2(355-1,AfeImut)-MCP-2xNLS-NES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Mutation

1 nucleotide removed in cdc2 promoter to abolish …

Promoter

Spom\P.cdc2

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pcdc2short-MCP-2xNLS-2xNES-tdSG

Plasmid

#255017

Purpose

expression of MCP-2xNLS-2xNES-tandemStayGold from medium-strength P.cdc2 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.cdc2(355-1,AfeImut)-MCP-2xNLS-2xNES-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Mutation

1 nucleotide removed in cdc2 promoter to abolish …

Promoter

Spom\P.cdc2

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUra4-Pmad3-stdMCP-NLS-tdSG

Plasmid

#255018

Purpose

expression of tandemMCP-NLS-tandemStayGold from weak P.mad3 promoter

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

P.mad3(717-1)-stdMCP-NLS-td8ox2StayGold-Scer\T.ADH1

Expression

Yeast

Promoter

Spom\P.mad3

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pSX018 BAC-phagemid CmR(TAG)

Plasmid

#253999

Purpose

BAC-phagemid for LySE, carrying CmR(TAG) for fluctuation analysis. Yeast origin (CEN) and selection (URA3) facilitates assembly in yeast.

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

CmR(TAG)

Expression

Yeast

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD097

Plasmid

#252986

Purpose

A MoClo Yeast Toolkit URA3, CEN/ARS empty vector (GFP dropout). Contains ConLS, part 234 GFP Dropout, ConR1, URA3, CEN6/ARS4, AmpR-CoIE1

Depositor

Paul Young

Publication

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

GFP

Expression

Yeast

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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mStay Gold Nat

Plasmid

#254395

Purpose

Plasmid for tagging C-terminal yeast proteins with mStay Gold with NatR marker, also has a 3xHA tag

Depositor

Aaron Hoskins

Insert

mStayGold

Expression

Yeast

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pJF87

Plasmid

#253754

Purpose

Encodes GFP_AID* and pALD6-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.

Depositor

Matthias Heinemann

Inserts

GFP_AID*
OsTIR1

Tags

mAID*-tag

Expression

Yeast

Mutation

F74G, S210A

Promoter

ALD6 and TEF1

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pPICZ:hTRAAK(W262S)

Plasmid

#130670

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Publication

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, W262S

Available since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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