8,173 results
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Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6L1TAG
Plasmid#158143PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertFAPB2.3.6L1TAG
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165V
Plasmid#158965PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165V
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165R
Plasmid#158966PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165R
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFapO_94
Plasmid#149597PurposepXI-3-LoxP-KlURA3-pTEF1- FapO_94-yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-FapO_94Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXylO_94
Plasmid#149598PurposepXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-XylO_94Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetO_105
Plasmid#149599PurposepXI - 3 -LoxP -KlURA3 -pTEF1 - TetO_105 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-TetO_105Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA271
Plasmid#149600Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_183 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA272
Plasmid#149601Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_182 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA197
Plasmid#149602Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterPcaO_1O8Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_79
Plasmid#149603Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - TetRDepositorInsertTetR
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyExpressionYeastPromoteract1Available SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL27-decoder
Plasmid#159449PurposeInsert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPfus1tet-citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_01
Plasmid#149606PurposepTEF1-VanR inserted in pRS416 backboneDepositorInsertVanR
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJPS2957
Plasmid#135591PurposeSNR6 has GAGAUUUAUUUC at position 96–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
ExpressionYeastMutationU6Δ12 has GAGAUUUAUUUC at position 96–107 deletedPromoterNativeAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG569
Plasmid#158588PurposetNCS-ePTS1 Cen6/ARS4DepositorInserttNCS-ePTS1
ExpressionYeastAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPL1B-Cl
Plasmid#160430PurposeExpresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoterDepositorInsertRPL1B
TagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to vary nucleoti…PromoterS. cerevisiae GAL1Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD217
Plasmid#160939PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mTagBFP2-HA Tag
TagsHA tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD118
Plasmid#160938PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mCherry-FLAG tag
TagsMYC tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD115
Plasmid#160937PurposeExcess Capsid Protein for in vivo mRNA displayDepositorInsertMCP-mCherry-MYC tag
TagsMYC tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD163
Plasmid#160935PurposeIn vivo mRNA display construct for mCherryDepositorInsertMCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1160c2
Plasmid#159724PurposeExpress MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MPGIL-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-VTS-US
Plasmid#160425PurposeExpression of c-luciferase from sp. Vargula tsujii (USA)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-MSVU-BZ
Plasmid#160426PurposeExpression of c-luciferase from sp. Maristella sp. SVU (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG1059
Plasmid#158595PurposetNCS-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)DepositorInsertstNCS-ePTS1
Ec_CYP80B1
Ps_6OMT
Ps_4'OMT2
Ps_CNMT
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG1060
Plasmid#158596PurposetNCS-dead-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)DepositorInsertstNCS-dead_ePTS1
Ec_CYP80B1
Ps_6OMT
Ps_4'OMT2
Ps_CNMT
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG1067
Plasmid#158597PurposetNCS-Venus (CEN6/ARS4)DepositorInserttNCS-Venus-ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJB207
Plasmid#158599PurposeUbiY-Venus-ePTS1DepositorInsertUbiY-Venus-ePTS1
TagsUbi-Y and ePTS1ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG1066
Plasmid#158590PurposetNCS-Venus-ePTS1 CEN6/ARS4DepositorInserttNCS-Venus-ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAS1643
Plasmid#158591PurposetNCS-VioE CEN6/ARS4DepositorInserttNCS-VioE-ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAS1644
Plasmid#158592PurposetNCS-VioE-dead_ePTS1 CEN6/ARS4DepositorInserttNCS-VioE-dead_ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only