8,060 results

1. pCfB3038(XII-1 MarkerFree)

   Plasmid

   #73278

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCfB3040(XII-4 MarkerFree)

   Plasmid

   #73280

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBW-m4046580

   Plasmid

   #145391

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. p41Hyg 3-F GW

   Plasmid

   #58555

   Purpose

   CEN vector with Hygromycin resistance marker for triple Gateway insertion

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB2799

   Plasmid

   #63658

   Purpose

   pCfB2799 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG303GPD-ccdB-HA

   Plasmid

   #14230

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDM3193

   Plasmid

   #113390

   Purpose

   Alpha factor inducible Ty3 with his3AI

   Depositor

   Suzanne Sandmeyer

   Article

   Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

   Insert

   Ty3-his3AI
   

   Use

   Tags

   his3AI

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV0816

   Plasmid

   #133510

   Purpose

   Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 12x_a1gRNAOp_pCYC1m_yeBFP2

   Plasmid

   #64393

   Purpose

   encodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced BFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pCYC1

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. GTH-c

    Plasmid

    #81103

    Purpose

    Gene Tagging vector HIS/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    yemCFP
    

    Use

    Cre/Lox

    Tags

    yemCFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBW-m4046255

    Plasmid

    #149040

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGBW-m4046341

    Plasmid

    #148981

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pPS2192

    Plasmid

    #8923

    Depositor

    Pamela Silver

    Article

    Hood et al J Cell Sci 2001 Feb;114(Pt 3):589-97.

    Insert

    Clb2-deltaNLS-GFP (CLB2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Deletion of CLB2 bipartite NLS (aa 183-200), KpnI…

    Promoter

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t)

    Plasmid

    #106465

    Purpose

    Expresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    YBR139W

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Gal4pBS34-Pleum

    Plasmid

    #60334

    Purpose

    Contains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. LD-BS-Trp1

    Plasmid

    #69503

    Purpose

    Improved version for complementation of yeast auxotrophic marker TRP1

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    TRP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p413GPD-hTPI Ile170Val

    Plasmid

    #50720

    Purpose

    expression of the human TPI Ile170Val allele in yeast

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 170 to Valine

    Promoter

    GPD

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS416 Gal-RNQ1 1-153-YFP

    Plasmid

    #18690

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 1-153
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    NonPrion domain of Rnq1

    Promoter

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pAG424GPD-ccdB-Cerulean

    Plasmid

    #14392

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB2198

    Plasmid

    #67554

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge