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pINER4R

Plasmid

#185884

Purpose

In vivo gene amplification (HapAmp) of nerolidol synthetic module locus (25 copy).

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP5EG3

Plasmid

#185878

Purpose

A plasmid for in vivo gene amplification (HapAmp) at SEC23 locus (1 copy reference)

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4BG6

Plasmid

#185877

Purpose

A parental plasmid for in vivo gene amplification (HapAmp) at SEC23 locus

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3AE4

Plasmid

#185873

Purpose

A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~11 copy)

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3A5C

Plasmid

#185872

Purpose

A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (1 copy reference)

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3A2 (2)

Plasmid

#185852

Purpose

Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGP8GF-AtTIR1

Plasmid

#188427

Purpose

Arabidopsis Nuclear Auxin Signaling Receptor TIR1

Depositor

Britney Moss

Publication

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)

Expression

Yeast

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ccdB

Plasmid

#188445

Purpose

Cloning Vector and pDEST

Depositor

Britney Moss

Publication

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

ccdB

Expression

Yeast

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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KBB520

Plasmid

#185102

Purpose

1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP

Depositor

Ken Belanger

Publication

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

POM152(1-200)-GFP

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB464

Plasmid

#185097

Purpose

HA-tagged POM152 with glycosylation sites mutated and with GFP at C-terminus

Depositor

Ken Belanger

Publication

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

Pom152DGlyc-GFP

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9337 (pgRNA_IV-1_NatMX)

Plasmid

#161590

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

Depositor

Irina Borodina

Publication

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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KBB418

Plasmid

#185088

Purpose

Swi6L-GFP with nuclear export signal mutated to alanines

Depositor

Ken Belanger

Publication

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 nuclear export signal L to A mutations

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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KBB439

Plasmid

#185093

Purpose

NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

Depositor

Ken Belanger

Publication

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

GAL1::NMA111-GFP

Promoter

GAL1

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP9E3

Plasmid

#185842

Purpose

Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB460

Plasmid

#185094

Purpose

NMA111-GFP wild type under endogenous promoter

Depositor

Ken Belanger

Publication

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

NMA111-GFP

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB285

Plasmid

#185076

Purpose

RFA3-GFP fusion

Depositor

Ken Belanger

Publication

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA3

Tags

GFP

Expression

Yeast

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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LDB107

Plasmid

#185423

Purpose

NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin

Depositor

Ken Belanger, Laura Davis

Publication

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

NUP1

Tags

HA

Expression

Yeast

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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KBB284

Plasmid

#185075

Purpose

RFA2-GFP fusion

Depositor

Ken Belanger

Publication

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA2

Tags

GFP

Expression

Yeast

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG

Plasmid

#176396

Purpose

Expresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.

Depositor

Matthew Good

Publication

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

GAL1 promoter, SZ1-RGG-GFP-RGG-RGG

Expression

Yeast

Promoter

GAL1

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK078

Plasmid

#171350

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

TxlnA ANIA_03613 terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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