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pTH737-CEN-maxHIS3

Plasmid

#38229

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Synthetic, Budding Yeast)

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Expression

Yeast

Promoter

SSA1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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6x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64392

Purpose

encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pTGPD-H-Hsp82

Plasmid

#1278

Depositor

Susan Lindquist

Article

Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

Insert

Hsp82 (HSP82 Budding Yeast)

Tags

Histidinex6

Expression

Yeast

Promoter

GPD

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pGBW-m4046696

Plasmid

#153882

Purpose

Yeast expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSP571

Plasmid

#139498

Purpose

CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD Cas9 / sgRNA (STL162)

Expression

Yeast

Mutation

WT

Promoter

pGPD

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTH399-SUP45-S30P

Plasmid

#29372

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

S30P mutant gene (corresponding to the sl23ts all…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pTH375-SUP45-P174Q

Plasmid

#29383

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

P174Q mutant gene including genomic sequence 1432…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pNB763

Plasmid

#60158

Purpose

Firefly luciferase (Promega) driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LYS9pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-URA3

Plasmid

#122894

Purpose

TRP1-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-HphMx

Plasmid

#122898

Purpose

LYS2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-KanMx

Plasmid

#122899

Purpose

LYS2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-NatMx

Plasmid

#122900

Purpose

LYS2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-HphMx

Plasmid

#122901

Purpose

TRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

Plasmid

#126739

Purpose

yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh2, acs1*, ald6

Tags

6xHis,Myc

Expression

Yeast

Mutation

T3542C and A30S, S629N (please see depositor comm…

Promoter

GAP

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAG423GPD-ccdB-ECFP

Plasmid

#14174

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Nup124-GFP-MTn

Plasmid

#154365

Purpose

Yeast expression of Nup124

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Nup124 (nup124 Fission Yeast)

Expression

Yeast

Mutation

See depositor comments below

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCH043

Plasmid

#47949

Purpose

rtTA under the control of GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pDH5

Plasmid

#83775

Purpose

Useful for tagging yeast proteins with C-terminal YFP tag; his selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Mutation

S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pNC952

Plasmid

#20401

Depositor

Beverly Errede

Article

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP-reporter

Expression

Yeast

Mutation

X=UbiM (see schematic) linker=deltak

Available Since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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