8,129 results

1. pAV0784

   Plasmid

   #133475

   Purpose

   Integration into fission yeast genome at his5 locus with bsdMX selection cassette

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG414GPD-ccdB-TAP

   Plasmid

   #14264

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   TAP

   Expression

   Yeast

   Available Since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE ADE2-HIS3

   Plasmid

   #122883

   Purpose

   ADE2-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBT9-chNfasc (+RSLE)

   Plasmid

   #89418

   Purpose

   Two-hybrid system

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   NFASC (NFASC Chicken)
   

   Tags

   GAL4 DBD

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046170

   Plasmid

   #149119

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pUC_delta ald1

   Plasmid

   #126708

   Purpose

   vector for knocking out ald1 in Komagataella phaffii

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   HIS4
   

   Expression

   Yeast

   Promoter

   His

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046247

   Plasmid

   #149124

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp2

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pXP212

   Plasmid

   #26828

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG415GPD-ccdB-EYFP

   Plasmid

   #14218

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPS311

    Plasmid

    #8903

    Depositor

    Pamela Silver

    Article

    Flach et al Mol Cell Biol. 1994 Dec . 14(12):8399-40

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pGBW-m4046415

    Plasmid

    #149035

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pGBW-m4046246

    Plasmid

    #145553

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pGBW-m4046455

    Plasmid

    #145487

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGBW-m4046231

    Plasmid

    #145451

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAG303GAL-ccdB-Cerulean

    Plasmid

    #14373

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT79_GalL_nCDA1Δ192-BE3

    Plasmid

    #145045

    Purpose

    Expresses nCDA1Δ192-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ192-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    pmCDA1(1-192AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXRT1

    Plasmid

    #63151

    Purpose

    Trp1 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

    Depositor

    Marc Gartenberg

    Article

    Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Bacterial and Yeast

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CDC12(K351N)

    Plasmid

    #1163

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    CDC12 (CDC12 Budding Yeast)
    

    Tags

    266bp of upstream sequence

    Expression

    Yeast

    Mutation

    K351N

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTH744-CEN-RLuc/slowmaxCFLuc

    Plasmid

    #38224

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS306Sup35R1+2

    Plasmid

    #17716

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    Sup35R1+2 (SUP35 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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