Skip to main content
Addgene
Showing: 3761 - 3780 of 7892 results

  4. pYC6Lys-TRP1URA3

    Plasmid
    #11010
    Depositor
    Francisco Malagon
    Insert
    Pgal1TRP1URA3
    Use
    Tags
    V5 and his6
    Expression
    Bacterial and Yeast
    Mutation
    ORF-TRP1 fused to ORF-URA without stop codon
    Promoter
    Available since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    11010-plasmid-map-sequence-id-6844

    Enlarge

  5. wt Cus2p

    Plasmid
    #73046
    Purpose
    Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    T7
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    73046-plasmid-map-sequence-id-150790

    Enlarge

  8. Hsh155 K740R

    Plasmid
    #111953
    Purpose
    This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740R mutation
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 K740R
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    K740R
    Promoter
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    111953-plasmid-map-sequence-id-213595

    Enlarge

  15. pAV113.loxP

    Plasmid
    #63186
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63186-plasmid-map-sequence-id-131462

    Enlarge

  18. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60164-plasmid-map-sequence-id-96373

    Enlarge

  19. pNB695

    Plasmid
    #60144
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60144-plasmid-map-sequence-id-96226

    Enlarge

  20. pNB697

    Plasmid
    #60146
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    RdLuc
    Use
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Mutation
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60146-plasmid-map-sequence-id-96253

    Enlarge

Showing: 3761 - 3780 of 7892 results