8,057 results
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Plasmid#64387Purposeencodes g1 gRNADepositorInsertg1 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c7gRNA_RPR1t
Plasmid#64385Purposeencodes c7 gRNADepositorInsertc7 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c1gRNA_RPR1t
Plasmid#64379Purposeencodes c1 gRNADepositorInsertc1 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c5gRNA_RPR1t
Plasmid#64383Purposeencodes c5 gRNADepositorInsertc5 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS13-Pleum
Plasmid#60332PurposeContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJW1509
Plasmid#62363PurposepFA6a BirA-heh2 linker-SSH1::HIS5DepositorInsertBirA-heh2-Ssh1
UseTagsExpressionYeastMutationPromoterGal1Available sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAD-hPLEKHM1
Plasmid#64145PurposeFor yeast two hybridDepositorInsertPLEKHM1 (PLEKHM1 Human)
UseTagsGAL4 activation domainExpressionYeastMutationPromoterADHAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAD-hRubicon
Plasmid#64144PurposeFor yeast two hybridDepositorInsertRubicon (RUBCN Human)
UseTagsGAL4 activation domainExpressionYeastMutationPromoterADH1Available sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB786
Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB762
Plasmid#60150PurposeFirefly luciferase (Promega) driven by LEU1 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB767
Plasmid#60159PurposeFirefly luciferase (Promega) driven by SIC1 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationA4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW3.31
Plasmid#62865PurposeYeast two-hybrid bridge vector expressing PAA-1DepositorInsertPAA-1/Y3H
UseTwo-hybrid bridge vectorTagsExpressionYeastMutationPromoterTEFAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2deltaC
Plasmid#60809Purposeto make truncation of yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationrev3ΔC encodes for Rev3 lacking the C-terminus (a…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2FeS
Plasmid#60808Purposeto disrupt iron sulfur cluster in yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationcontains 647 C-terminal amino acids and 335 bp of…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS134-Pleum
Plasmid#60339PurposeContains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS3-Pleum
Plasmid#60330PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS1-Pleum
Plasmid#59993PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS2-Pleum
Plasmid#59992PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
p414CYC_Hsp82_CycTER
Plasmid#61713Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterCYC1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414TEF_Hsp82_noTER
Plasmid#61714Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterTEF1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414ADH_Hsp82_noTER
Plasmid#61715Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterADH1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414CYC_Hsp82_noTER
Plasmid#61716Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterCYC1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C
Plasmid#60394PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptideDepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C
Plasmid#60398PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB7 Cterminal tail
Plasmid#60399PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB8 Cterminal tail
Plasmid#60401PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail
Plasmid#60402PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C
Plasmid#60405PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB693
Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
p426 3-F GW
Plasmid#58556PurposepRS426 carrying Gateway cassette for three insertsDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB649
Plasmid#60136PurposeFirefly luciferase (Promega) driven by MET17 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-CU1-Pleum
Plasmid#60331PurposeContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNB689
Plasmid#60133PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB756
Plasmid#60149PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterLEU1prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB752
Plasmid#60155PurposeGreen beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterADE17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB812
Plasmid#60160PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.DepositorInsertyEVenus-PEST
UseTagsPEST degronExpressionYeastMutationPromoterRNR1prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ286
Plasmid#52227PurposeExpression of sgRNA precursor targeted against ade6L469DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
p41Nat 3-F GW
Plasmid#58553PurposeCEN vector with clonNat resistance marker for triple Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Skn7t-L-IFP-F[1] (Yeast)
Plasmid#52896PurposeSkn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.DepositorInsertSkn7t-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1Available sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only