8,060 results

1. pCfB2991

   Plasmid

   #63643

   Purpose

   pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTG005

   Plasmid

   #71386

   Purpose

   localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast

   Depositor

   Mark Schmitt

   Article

   Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

   Insert

   RMP1 (RMP1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   ACT1

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB116

   Plasmid

   #53365

   Purpose

   To express Cup9p‐FLAG from the PGAL1 promoter

   Depositor

   Alexander Varshavsky

   Article

   Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

   Insert

   Cup9 (CUP9 Budding Yeast)
   

   Use

   Tags

   Flag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pRS413 Prp5 WT

   Plasmid

   #87599

   Purpose

   Encodes Prp5 WT for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   PRP5 (PRP5 )
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ube2i

   Plasmid

   #31420

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBE2I Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pNB789

   Plasmid

   #60166

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   RNR1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG423GAL-ccdB-TAP

   Plasmid

   #14269

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   TAP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBT9-hSyntenin2b

   Plasmid

   #89422

   Purpose

   Two-hybrid system

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Yeast

   Mutation

   * see below

   Promoter

   ADH1

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)

   Plasmid

   #14846

   Depositor

   Erin O'Shea

   Article

   Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

   Insert

   PHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
   

   Use

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   TATA-A1T6. TATA box mutated from TATATAAG to AATA…

   Promoter

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pDS297

    Plasmid

    #34986

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    cpPDZ
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ube2h

    Plasmid

    #31419

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2H Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    *see comments

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Ube2L3

    Plasmid

    #31421

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2L3 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pLUK

    Plasmid

    #64184

    Purpose

    harbours marker genes LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

    Plasmid

    #68274

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. YIp211-P30-His-pol30(K127R)-3'

    Plasmid

    #99547

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNXRVat-HACre

    Plasmid

    #78399

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    TKanAX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNXRVbt-HACre

    Plasmid

    #78402

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    HyTkAX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GTL-ir

    Plasmid

    #81102

    Purpose

    Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    tdiRFP
    

    Use

    Cre/Lox

    Tags

    tdiRFP

    Expression

    Yeast

    Mutation

    codon optimized second iRFP to avoid internal rec…

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. GTT-y

    Plasmid

    #81110

    Purpose

    Gene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCitrine
    

    Use

    Cre/Lox

    Tags

    mCitrine

    Expression

    Yeast

    Mutation

    Mutated mCitrine for monomeric isoform (A206K L22…

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. GTT-ir

    Plasmid

    #81112

    Purpose

    Gene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    tdiRFP
    

    Use

    Cre/Lox

    Tags

    tdiRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge