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pMM0617

Plasmid

#128993

Purpose

loxP-KIURA3-loxP preassembled entry vector

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0624

Plasmid

#128999

Purpose

pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAV10.F6.loxP

Plasmid

#63201

Purpose

pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

Depositor

Jef Boeke

Publication

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Oct. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Publication

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0621

Plasmid

#128996

Purpose

pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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G30, Cdc20-mCherry-AID tagging

Plasmid

#130270

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Publication

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMV14

Plasmid

#128861

Purpose

Position 1 module -2 microns

Depositor

Gilles Charvin

Publication

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

2 microns

Use

Synthetic Biology

Expression

Yeast

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFA CRU HISMX

Plasmid

#131164

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Publication

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

CRU

Expression

Yeast

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFA NuiHA-loxP-nat-CUP1p

Plasmid

#131170

Purpose

PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

Depositor

Nils Johnsson

Publication

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1671

Plasmid

#112045

Purpose

CEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ORM1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMV13

Plasmid

#128864

Purpose

Position 1 module- CEN (Centromeric)

Depositor

Gilles Charvin

Publication

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

CEN/ARS

Use

Synthetic Biology

Expression

Yeast

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA053

Plasmid

#128515

Purpose

Expresses SFA8 in Pichia

Depositor

Marcos Alcocer

Publication

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

SFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)

Expression

Yeast

Promoter

AOX1

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV12

Plasmid

#128893

Purpose

Position 6 module- NatMX

Depositor

Gilles Charvin

Publication

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

NatMX

Use

Synthetic Biology

Expression

Yeast

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*

Plasmid

#126739

Purpose

yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh2, acs1*, ald6

Tags

6xHis,Myc

Expression

Yeast

Mutation

T3542C and A30S, S629N (please see depositor comm…

Promoter

GAP

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des1

Plasmid

#110212

Purpose

Yeast Surface Display of G6des1

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des1

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA151

Plasmid

#128526

Purpose

Expresses mutant Ber e 1 in Pichia

Depositor

Marcos Alcocer

Insert

mutant Ber e 1

Expression

Yeast

Promoter

AOX1

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-CPR

Plasmid

#126742

Purpose

yeast plasmid overexpressing CPR

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

cpr

Tags

6xHis

Expression

Yeast

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-eGFP

Plasmid

#126720

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Tags

6xHis,Myc

Expression

Yeast

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-LacI-Mit1AD

Plasmid

#126722

Purpose

LacI-Mit1AD on pGAPZ_ A expression vector

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Expression

Yeast

Promoter

GAP

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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