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pDRF1-GW yosfGFP-T2A-mCherry

Plasmid

#118448

Purpose

Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yosfGFP-T2A-mCherry

Expression

Yeast

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW eGFP-T2A-mCherry

Plasmid

#118422

Purpose

Produces equimolar expression of eGFP and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

eGFP-T2A-mCherry

Expression

Yeast

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB4613

Plasmid

#126752

Purpose

Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker

Depositor

Michael Jensen

Publication

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

CDC14 (CDC14 Budding Yeast)

Use

Integrative plasmid

Expression

Yeast

Promoter

pTRX2_5xUAS

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMST-1312

Plasmid

#118075

Purpose

pTPI1-cexA-tCYC1

Depositor

Michael Sauer

Publication

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTPI1-cexA-tCYC1

Expression

Yeast

Promoter

pTPI1

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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wt Cus2p

Plasmid

#73046

Purpose

Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

Depositor

Aaron Hoskins

Publication

Rodgers et al RNA. 2015 Dec 2.

Insert

CUS2p (CUS2 Budding Yeast)

Tags

6x His tag fused to TEV cleavage site

Expression

Bacterial and Yeast

Promoter

T7

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1837

Plasmid

#123058

Purpose

_-factor pheromone

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

_-factor [3-4a]

Expression

Yeast

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCuHA-ATG13[3SA](426)

Plasmid

#122076

Purpose

Expressing HA-ATG13[3SA]

Depositor

Daniel Klionsky

Publication

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1(406)

Plasmid

#122068

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Publication

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pWS939

Plasmid

#123051

Purpose

Pheromone responsive transcription factor

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste12 [3]

Expression

Yeast

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWS627

Plasmid

#123064

Purpose

G___ bicistronic protein

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste4-2A-Ste18 [3-4a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS788

Plasmid

#123059

Purpose

_-factor protease

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Bar1 [3]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS936

Plasmid

#123050

Purpose

G_ subunit for creating C-terminal transplants

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 C-terminal Truncation Vector [3-4a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS625

Plasmid

#123046

Purpose

Adenosine GPCR

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

A2BR [3]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS067

Plasmid

#123044

Purpose

_-factor GPCR

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste2 [3]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRC246

Plasmid

#123038

Purpose

Core promoter

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pLEU2m [2b]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS484

Plasmid

#123032

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (2x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS483

Plasmid

#123031

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (3x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS482

Plasmid

#123030

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (4x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS053

Plasmid

#123025

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFIG1 [2]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS060

Plasmid

#123024

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFUS1 [2]

Expression

Yeast

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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