7,942 results

1. pH3FS-GAL1

   Plasmid

   #85782

   Purpose

   N-ICE plasmid pH3FS-GAL1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-HphMX-loxP-GAL1p-3FLAG
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL90-Nat-2xHO

   Plasmid

   #139069

   Purpose

   CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Insert

   Two guide RNAs targeting the HO endonuclease
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TDH3

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCT

   Plasmid

   #60620

   Purpose

   Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPS1372

   Plasmid

   #1353

   Depositor

   Pamela Silver

   Article

   Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

   Insert

   pADH-NLS-NES-GFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

5. 3019_pETcon-SARS-CoV-2-RBD_E484K

   Plasmid

   #184404

   Purpose

   yeast surface display of the SARS-CoV-2 Eta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   E484K

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG303GAL-ccdB-EGFP

   Plasmid

   #14181

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREMI-Z

   Plasmid

   #59527

   Purpose

   Zeocin-resistance plasmid for insertional mutagenesis.

   Depositor

   Benjamin Glick

   Article

   Mukaiyama et al Genes Cells. 2002 Jan;7(1):75-90.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYES2-gRNA-hyg-MCS

   Plasmid

   #107734

   Purpose

   E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   X-3 site gRNA expression cassettes
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52p

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LD201

   Plasmid

   #69178

   Purpose

   Integration of 2GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg1-2EGFP
   

   Use

   Tags

   2xEGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYC61

    Plasmid

    #63925

    Purpose

    Recipient replicative vector to construct CFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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