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POM43-mCherry

Plasmid

#69857

Purpose

N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Expression

Yeast

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTH671-SUP35-H348Q

Plasmid

#29732

Depositor

Tobias von der Haar

Insert

SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)

Expression

Yeast

Mutation

H348Q mutant gene of SUP35 including genomic sequ…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 Q331K

Plasmid

#27461

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pRS315Nab3

Plasmid

#110532

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2903(XI-2 MarkerFree)

Plasmid

#73275

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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POM42-mTFP1

Plasmid

#69852

Purpose

N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMLB10

Plasmid

#177273

Purpose

GAL1p:T7RNAP(F11L;T613A):CYC1t

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

T7RNAP(F11L;T613A)

Expression

Bacterial and Yeast

Mutation

F11L T613A

Promoter

GAL1

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFS119

Plasmid

#12379

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

ade6, thymidine kinase

Expression

Yeast

Available Since

Sept. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pIM572

Plasmid

#74640

Purpose

alias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

none

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pROS14

Plasmid

#107928

Purpose

LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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