7,943 results

1. pFA0055

   Plasmid

   #131774

   Purpose

   Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer CASS5a guide (gCASS5a) and 5' sgRNA
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HsSPHK2

   Plasmid

   #118092

   Purpose

   Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, 10

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG416GALL-HAV_3C

   Plasmid

   #203481

   Purpose

   Expresses HAV 3C protease from a GALL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Insert

   HAV 3C protease
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GALL

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PSUC2-yEGFPCLN2PEST-pRS406

   Plasmid

   #64405

   Purpose

   SUC2 promoter driven GFP expression

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   SUC2 promoter (SUC2 Budding Yeast)
   

   Use

   Tags

   yEGFPCLN2PEST (destabilized yeast EGFP)

   Expression

   Yeast

   Mutation

   Promoter

   SUC2

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS10

   Plasmid

   #83795

   Purpose

   Useful for tagging yeast proteins with C-terminal Cerulean tag; hygromycin-B selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Cerulean
   

   Use

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-KanMX6-Purg1-3FLAG

   Plasmid

   #19354

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Aug 26. ():.

   Insert

   urg1 promoter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1667

   Plasmid

   #112041

   Purpose

   CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   ERG5
   

   Use

   Tags

   V5-TEV-EGFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSK234

   Plasmid

   #131149

   Purpose

   dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPKC1 (JCE575)

   Plasmid

   #89773

   Purpose

   yeast expression of PKC1

   Depositor

   Juan Carlos Igual

   Article

   Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

   Insert

   PKC1 (PKC1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   yeast PKC

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAST BmOr2

    Plasmid

    #73926

    Purpose

    UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    BmOr2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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