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pFBP-2_6m.mutant

Plasmid

#162842

Purpose

Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6m_RNA-device

Expression

Yeast

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pML104-natR412

Plasmid

#162042

Purpose

Expresses Cas9 and contains natR412 guide which targets the natR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

natR412 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pML107-natR854

Plasmid

#162043

Purpose

Expresses Cas9 and contains natR854 guide which targets the natR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

natR854 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDN513

Plasmid

#175365

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, HIS3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN514

Plasmid

#175366

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, TRP1-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN515

Plasmid

#175367

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, LEU2-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN516

Plasmid

#175368

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, URA3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN523

Plasmid

#175369

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN524

Plasmid

#175370

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, TRP1-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN525

Plasmid

#175371

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN526

Plasmid

#175372

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN613

Plasmid

#175373

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, HIS3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN614

Plasmid

#175374

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, TRP1-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN615

Plasmid

#175375

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, LEU2-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN616

Plasmid

#175376

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, URA3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN623

Plasmid

#175377

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN624

Plasmid

#175378

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, TRP1-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN625

Plasmid

#175379

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDN626

Plasmid

#175380

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p406 – TDH3-ER-FlucDM-GFP11-Ura

Plasmid

#177731

Purpose

DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

FlucDM

Tags

GFP11, HA, HDEL, and KAR2 Signal Sequence

Expression

Bacterial and Yeast

Mutation

R188Q, R261Q (Destabilized Mutant)

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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