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pG1-NMGR526

Plasmid

#1122

Depositor

Susan Lindquist

Article

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

sup35 NM domain fused with GR (SUP35, Nr3c1 Rat, Budding Yeast)

Tags

GR526

Expression

Yeast

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only
p416TEF-NYFP

Plasmid

#99553

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pNC891

Plasmid

#20397

Depositor

Beverly Errede

Article

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

YFP-reporter

Expression

Yeast

Mutation

X=M (see schematic)

Available Since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pAS1

Plasmid

#12388

Depositor

Nick Rhind

Article

Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

Insert

green fluorescent protein

Expression

Yeast

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pVEG17*

Plasmid

#111856

Purpose

Xylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)

Depositor

Nikhil Nair

Article

Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

Insert

GAL1p-GAL3-Syn4.1-TEFt

Use

Synthetic Biology

Expression

Yeast

Mutation

D68N, V69M, A109V, I271L

Promoter

GAL1p

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB3049(gRNA XII-4)

Plasmid

#73291

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-2TAG

Plasmid

#158138

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Synthetic, Chicken)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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YCplac111-yVps23-mCherry

Plasmid

#45925

Depositor

James Hurley

Article

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23 (STP22 Budding Yeast)

Tags

mCherry

Expression

Yeast

Promoter

lac

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only