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pDRF1-GW yeVenus-T2A-mTurquoise2

Plasmid

#118440

Purpose

Produces equimolar expression of yeVenus and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yeVenus-T2A-mTurquoise2

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW tagRFP-T2A-mTurquoise2

Plasmid

#118437

Purpose

Produces equimolar expression of tagRFP and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

tagRFP-T2A-mTurquoise2

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW mTurquoise2-T2A-mcherry

Plasmid

#118433

Purpose

Produces equimolar expression of mTq2 and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mTurquoise2-T2A-mcherry

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW Clover-T2A-mTurquoise2

Plasmid

#118425

Purpose

Produces equimolar expression of Clover and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

Clover-T2A-mTq2

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-ATG13[3SD](315)

Plasmid

#122074

Purpose

Expressing GFP-Atg13[3SD]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Mutation

Serine 646 to aspartic acid; Serine 649 to aspart…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-ATG13[3SA](315)

Plasmid

#122073

Purpose

Expressing GFP-Atg13[3SA]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

GFP

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCuHA-SpAtg17(416)

Plasmid

#122079

Purpose

Expressing S. pombe HA-Atg17

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Tags

3HA

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCuHA-SpAtg101(414)

Plasmid

#122081

Purpose

Expressing S. pombe HA-Atg101

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG101

Tags

3HA

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1-ATG7 3'UTR(406)

Plasmid

#122070

Purpose

Expressing ATG1 with ATG7 3'UTR

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native 3'UTR was replaced by ATG7 3'…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-SpAtg17(416)

Plasmid

#122078

Purpose

Expressing S. pombe Atg17

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS-ATG7p-ATG1(406)

Plasmid

#122071

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native promoter was replaced by ATG7 promoter…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS-Dhh1-PA(D195A,E196A)(405)

Plasmid

#122065

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(S226A,T228A)(405)

Plasmid

#122066

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Serine 226 to alanine; Threonine 228 to alanine

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(F317D, I325A)

Plasmid

#122275

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317H)

Plasmid

#122269

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Histidine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(I325A)

Plasmid

#122274

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317D, I325A)

Plasmid

#122273

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(I325A)

Plasmid

#122272

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317Q)

Plasmid

#122271

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to glutamine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(F317D)

Plasmid

#121391

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge