8,400 results
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Plasmid#222485PurposeCo-expression of two genes in yeast with V5 and HA tags and URA3 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only
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pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS415-GPD
Plasmid#183940PurposeControl vector that has a GPD promoter with no insertDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterENO1pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBP-2_6.sensor
Plasmid#162800PurposeYeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6DepositorInsert2_6_RNA-device
ExpressionYeastAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p
Plasmid#158141PurposeExpresses Aga1p in yeastDepositorInsertAga1p
ExpressionYeastPromoterGAL10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarletI-KanMX6
Plasmid#136690PurposeTagging genes in Cterm with mScarletI in fission yeastDepositorInsertmScarletI
TagsmScarletIExpressionYeastPromoterNoneAvailable SinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM27
Plasmid#133641PurposeIntegration of GFP-PHO8 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast vacuole.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME4390
Plasmid#129656PurposeLEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-CRY2-uTEV1Δ(220-242)
Plasmid#135452PurposeYeast expression of uTEV1Δ fused to CRY2 and mCherryDepositorInsertmCherry-CRY2-TEV1Δ(220-242)
TagsmCherryExpressionYeastPromoterGalAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0133
Plasmid#133467PurposeIntegration into fission yeast genome at ura4 locusDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C1
Plasmid#125549PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to c-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C2
Plasmid#125559PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to c-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-HDEL
Plasmid#129098PurposeFor labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDELDepositorInsertTEF1p-SS-3xGlyAla-GFP-HDEL
ExpressionYeastAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKL05
Plasmid#104429Purposesuper Ecliptic pHluorin in yeast expression vectorDepositorInsertSuper Ecliptic pHluorin
ExpressionYeastPromoterTef1Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mCherry
Plasmid#125700PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mCherryDepositorInsertymNeongreen-T2A-mCherry
ExpressionYeastAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTurquoise2
Plasmid#118453PurposeOverexpression of yeast codon-optimized mTq2DepositorInsertymTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH243
Plasmid#121147PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeastDepositorInsertshumanized Streptococcus pyogenes Cas9
gRNA expression module
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPH1-mEGFP
Plasmid#105270PurposePlasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.DepositorAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only