8,432 results
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Plasmid#89191PurposeExpresses YFP under control of GAL1 promoter.DepositorInsertpGAL1-YFP
ExpressionYeastPromoterGAL1Available sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9v2
Plasmid#103140PurposeTetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready siteDepositorInsertdCas9-VPR
ExpressionYeastMutationRemoved scaffold gRNA from original plasmidAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRA03
Plasmid#103141PurposeTriple gRNA expression separated with 28nt Csy4 motifDepositorInsertgRNAs
ExpressionYeastAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDE710
Plasmid#103020Purposeexpression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)DepositorAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YIp204-PADH1-AFB2
Plasmid#99531PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK005-3a-αMF
Plasmid#84964PurposeType 3a, α-mating factor - secretion signalDepositorInsertα-mating factor secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.Available sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
ExpressionYeastPromoterGAL1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
ExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
TagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2797
Plasmid#63639PurposepCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCfB2796
Plasmid#63641PurposepCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIM691
Plasmid#74641Purposealias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection markerDepositorInsertymTagBFP
ExpressionYeastPromoternoneAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2189
Plasmid#67532PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PhyB-mCherry-Tom7
Plasmid#66571PurposeConstitutively express mitochondrial localized PhyB in Saccharomyces cerevisiaeDepositorPublicationInsertPhyB (PHYB Mustard Weed)
TagsTom7 and mCherryExpressionYeastMutationPhyB portion contains aa's 1-908PromoterpADH1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3Kl
Plasmid#69195Purposecomplementation of yeast auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertKluyveromyces lactis URA3
ExpressionYeastAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DdMCU
Plasmid#67642PurposeDdMCU-Flag in pACT2 vector human codon optimisedDepositorInsertDdMCU
TagsFlag tag with linkerExpressionYeastPromoterADH1Available sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG216
Plasmid#64528PurposeCentromeric E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG201
Plasmid#64394PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC595
Plasmid#62322PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
TagsVP64ExpressionYeastMutationHas nuclease-inactivating mutations in the RuvC1 …PromoterTdh3 and pAdhAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by