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pKmK.T4

Plasmid

#125056

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Publication

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PDC1 terminator

Use

Synthetic Biology

Expression

Yeast

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymYPET-CaURA3

Plasmid

#118460

Purpose

Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-ytdTomato-CaURA3

Plasmid

#118461

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

Plasmid

#117662

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Publication

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pOst1-pro-af(MUT1)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVG1

Plasmid

#111444

Purpose

Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

Depositor

Gerald Fink

Publication

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2/stop-codon repair

Use

CRISPR

Expression

Yeast

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFA6a- mYFP-kanMX6

Plasmid

#105147

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-kanMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mMaple3-kanMX6

Plasmid

#105165

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

mMaple3-kanMX6

Tags

GlySerGly-mMaple3

Expression

Bacterial and Yeast

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pXNubWtgate

Plasmid

#105080

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubI

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-msCFPx6

Plasmid

#105268

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Tags

msCFPx6

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMetOYC-Dest

Plasmid

#105082

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning

Depositor

Christopher Grefen

Publication

Xing et al Plant Physiol. 2016 Jun;171(2):727-58. d

Type

Empty backbone

Tags

Cub-PLV and mOST4

Expression

Yeast

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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dCas9v2

Plasmid

#103140

Purpose

TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

Depositor

Jens Nielsen

Publication

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

dCas9-VPR

Expression

Yeast

Mutation

Removed scaffold gRNA from original plasmid

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Publication

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE710

Plasmid

#103020

Purpose

expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YIp204-PADH1-AFB2

Plasmid

#99531

Purpose

S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

Depositor

Helle Ulrich

Insert

AFB2 (AFB2 Mustard Weed)

Expression

Yeast

Promoter

ADH1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK005-3a-αMF

Plasmid

#84964

Purpose

Type 3a, α-mating factor - secretion signal

Depositor

Volker Sieber

Publication

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ cMyc Dam_f_GFPoNLS

Plasmid

#85820

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Publication

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc Dam_f_GFPoNLS

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A.

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPB5A

Plasmid

#83556

Purpose

Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

GAL1 promoter-yEGFP

Expression

Yeast

Promoter

GAL1

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC-TALO8

Plasmid

#83409

Purpose

TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

Depositor

Toshi Tsukiyama

Publication

Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

Insert

TRP-ARS-8xLacO

Expression

Yeast

Mutation

Please see depositor comments below

Promoter

TRP1

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS406-CMV-lacI-3FLAG

Plasmid

#83410

Purpose

Expression of lacI in yeast

Depositor

Toshi Tsukiyama

Publication

Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

Insert

lacI repressor

Tags

3xFLAG and NLS

Expression

Yeast

Promoter

CMV

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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