8,350 results
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Plasmid#22316DepositorInsertGFP-rad9-GFP
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(URA)
Plasmid#49460Purposeoverexpression of Flp step-arrest mutant in yeast, URA selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
ExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available SinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTIN-ura4
Plasmid#161808PurposeA ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombeDepositorTypeEmpty backboneExpressionYeastPromoterPho1Available SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE2n
Plasmid#17456Purposemodified pENTR vector for N-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pESC-HIS (V5 and HA)
Plasmid#222487PurposeCo-expression of two genes in yeast with V5 and HA tags and HIS3 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX122-mtGFP
Plasmid#45048DepositorInsertmtGFP
ExpressionYeastPromoterTPIAvailable SinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
ExpressionYeastPromoterTDH3Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-YFP-yeastSMASh
Plasmid#68855PurposeExpresses SMASh tag fused to YFP at C terminus in yeastDepositorInsertYFP-yeastSMASh
TagsSMASh tagExpressionYeastPromoterGPDAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNubWtXgate
Plasmid#105079PurposeYeast mating-based Split Ubiquitin Assay, positive controlDepositorTypeEmpty backboneTagsNubIExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Strep-tagII-kanMX6
Plasmid#20757DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pREP3xV5_ccdB
Plasmid#79015PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_nat
Plasmid#184911PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXP842
Plasmid#46068DepositorTypeEmpty backboneExpressionYeastPromoterADH2Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationA4VPromoterMET17prAvailable SinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA E171Q GFP
Plasmid#32127DepositorAvailable SinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
TagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV0356
Plasmid#133468PurposeIntegration into fission yeast genome at ade6 locusDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM151
Plasmid#133643PurposeIntegration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSCupSup35GFP
Plasmid#1087DepositorAvailable SinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only