8,431 results
-
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
ExpressionYeastPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS35
Plasmid#83797PurposeUseful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selectionDepositorPublicationInsertmCherry
TagsmCherryExpressionYeastAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHLU
Plasmid#64167Purposeharbours marker genes HIS3, LEU2 and URA3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAV123
Plasmid#63189PurposepRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PP74
Plasmid#78944PurposeSupplemental Figure 3. Integration Site 1.DepositorInsertIntegration Site 1
ExpressionYeastAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415GPD-3xHA-GW
Plasmid#63781Purposeyeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insertDepositorTypeEmpty backboneTags3xHAExpressionYeastPromoterGPDAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416gT-GalL-Cas9
Plasmid#79903PurposepRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expressionDepositorInsertsCas9
guide RNA (gRNA)
Tetracycline Repressor
UseCRISPRExpressionYeastPromoterGPM1 promoter, GalL, and RPR1 promoter with TetO …Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorPublicationInsertRGR-r10
UseCRISPR and Synthetic BiologyExpressionYeastPromoterADH1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD6-pGRR-r11-r10-yeGFP
Plasmid#74368PurposepGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.DepositorPublicationInsertyeGFP
UseCRISPR and Synthetic BiologyExpressionYeastPromoterpGRR-r11-r10Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188
Plasmid#67296PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW
Plasmid#64745PurposeYeast expression plasmid: ACC1DepositorInsertACC1
ExpressionYeastAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG207
Plasmid#64530PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP1483
Plasmid#64511PurposeExpresses destabilized Ametrine in yeast.DepositorInsertAmetrine-ADH1-tail
ExpressionYeastPromoterTDH3Available sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE
Plasmid#67293PurposeInduce human iN conversion from fibroblastDepositorAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx2(yeast)
Plasmid#65005PurposeYeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2DepositorInsertGRX2
TagsroGFP2ExpressionYeastAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC545
Plasmid#62313PurposesgRNA with 1x MS2 for yeast cellsDepositorInsertsgRNA + 1x MS2 binding module
ExpressionYeastPromoterSNR52Available sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYC54
Plasmid#63909PurposeRecipient integrative vector to construct YFP tagged fusionsDepositorTypeEmpty backboneTagsYFPExpressionYeastAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB648
Plasmid#60140PurposeNanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.DepositorInsertNLuc
TagsNuclear localization signal (NLS)ExpressionYeastPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB778
Plasmid#60154PurposeGreen click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBG99
ExpressionYeastPromoterLEU1prAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by