8,057 results

1. pHpgRNA13

   Plasmid

   #160127

   Purpose

   To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

   Depositor

   Yongjin Zhou

   Article

   Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

   Insert

   PTEF1-HH-gRNA-ADE2- HDV-TAMO
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUDC175

   Plasmid

   #103019

   Purpose

   S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   Fncpf1
   

   Use

   Tags

   3HA and NLS

   Expression

   Yeast

   Mutation

   human codon optimized

   Promoter

   TEF1

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDS220

   Plasmid

   #34980

   Purpose

   Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb–mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. BB3nK_ext_AD

   Plasmid

   #108687

   Purpose

   Plasmid for assembly of donor DNA templates consisting of three expression units

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSP-G1

   Plasmid

   #64736

   Purpose

   Yeast expression plasmid

   Depositor

   Jens Nielsen

   Article

   Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pESC-URA::CYP71AV1/CPR

   Plasmid

   #20118

   Depositor

   Jay Keasling

   Article

   Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

   Insert

   amorphadiene oxidase and p450 reductase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 2, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pDRF1-GW ymTurquoise2

   Plasmid

   #118453

   Purpose

   Overexpression of yeast codon-optimized mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS263

   Plasmid

   #122379

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMZ379

   Plasmid

   #74215

   Purpose

   Combined sgRNA/Cas9 vector with NatMX marker

   Depositor

   Mikel Zaratiegui

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIM23

    Plasmid

    #74643

    Purpose

    Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry

    Plasmid

    #203656

    Purpose

    Yeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Tags

    yEmCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pESC-ARG (V5 and HA)

    Plasmid

    #222493

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and ARG1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pESC-MET

    Plasmid

    #222482

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pESC-LYS

    Plasmid

    #222483

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pESC-TYR

    Plasmid

    #222484

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and TYR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pESC-MET (V5 and HA)

    Plasmid

    #222490

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and MET15 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pESC-LYS (V5 and HA)

    Plasmid

    #222491

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and LYS2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pESC-TYR (V5 and HA)

    Plasmid

    #222492

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and TYR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pESC-THR (V5 and HA)

    Plasmid

    #222494

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and THR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pESC-THR

    Plasmid

    #222479

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and THR1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge