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pRS416-PHO5-GFP-Osh1

Plasmid

#58835

Purpose

Expresses GFP-Tagged Osh1 in yeast

Depositor

Tim Levine

Publication

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Osh1 (SWH1, OSH1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-2xOsh2 PH domain [short]

Plasmid

#58829

Purpose

Expresses GFP-Tagged Osh2 PH domain in yeast

Depositor

Tim Levine

Publication

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2xOsh2 PH domain [short] (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
D771 leu2::LYS2 Disruptor Converter

Plasmid

#51677

Purpose

Yeast marker swap plasmid for converting LEU2 to LYS2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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p413TEF-Sir2

Plasmid

#51742

Purpose

overexpression of Sir2 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Publication

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Expression

Yeast

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-12Pk-ura4MX6

Plasmid

#49180

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

12Pk

Expression

Yeast

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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PRS415-Gal1pr-Mag1-sctetR

Plasmid

#44752

Depositor

Narendra Maheshri

Publication

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

MAG1-sctetR

Expression

Yeast

Promoter

Gal1pr

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pXP741

Plasmid

#46059

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP841

Plasmid

#46067

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yECitrine-Hygro

Plasmid

#44646

Depositor

Kurt Thorn

Insert

yECitrine

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yECFP-Hygro

Plasmid

#44647

Depositor

Kurt Thorn

Insert

yEmCFP

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yEPAGFP-CaUra3

Plasmid

#44649

Depositor

Kurt Thorn

Insert

yEPAGFP

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-T2dMFot

Plasmid

#44558

Depositor

Gabor Balazsi

Publication

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
rtetR-M2::FFF

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

Promoter

PTETREG

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-G1Gh

Plasmid

#44507

Depositor

Gabor Balazsi

Publication

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-AVT1 chr I

Plasmid

#41898

Purpose

Yeast expression of AVT1.

Depositor

Dan Gottschling

Publication

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

AVT1 (AVT1 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pZHY500

Plasmid

#36186

Depositor

Daniel Voytas

Publication

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

FokI homodimeric Nuclease domain

Tags

ACV5

Expression

Yeast

Mutation

delta152,+63 truncation to TAL backbone

Promoter

TEV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pDS244

Plasmid

#34982

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Ste11 (STE11 Budding Yeast)

Expression

Yeast

Promoter

ADH

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pTSK368

Plasmid

#34551

Depositor

James McNally

Publication

Karpova et al Science. 2008 Jan 25;319(5862):466-9.

Insert

MS2-CP-NLS

Tags

GFP

Expression

Yeast

Promoter

Met25

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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LHP462 - Ub-HA

Plasmid

#32169

Depositor

Linda Hicke

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Tags

HA

Expression

Yeast

Promoter

CUP1

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
LPH2781 - Ub-K63R

Plasmid

#32160

Depositor

Linda Hicke

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Mutation

K63R

Promoter

CUP1

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
LHP306 - Ub - no Lys

Plasmid

#32150

Depositor

Linda Hicke

Publication

Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Mutation

No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

Promoter

CUP1

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only