8,057 results

1. pESC-ADE

   Plasmid

   #222480

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and ADE2 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pESC-ARG

   Plasmid

   #222481

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and ARG1 selection

   Depositor

   Tetsuo Matsuzaki, Kiyofumi Yamada

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ymTq2-yeVenus pDRF1-GW

   Plasmid

   #168076

   Purpose

   FRET fusion between ymTq2 and yeVenus for use in yeast

   Depositor

   Bas Teusink

   Insert

   ymTq2-yeVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRF1-GW ymVenus

   Plasmid

   #118454

   Purpose

   Overexpression of yeast codon-optimized mVenus

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-G9-5FLAG-hphMX6

   Plasmid

   #49185

   Purpose

   genomic G9-5FLAG epitope tagging in S. pombe

   Depositor

   Eishi Noguchi

   Article

   Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

   Type

   Empty backbone

   Use

   Tags

   G9-5FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTH732-2µ-RLuc/staCFLuc

   Plasmid

   #40607

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   The last three codons of the full-length Firefly …

   Promoter

   ADH1 and TDH3

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYZ301

   Plasmid

   #102690

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-ura4MX6

   Plasmid

   #49184

   Purpose

   gene deletion in S. pombe

   Depositor

   Eishi Noguchi

   Article

   Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRR-AR-5Z

   Plasmid

   #23058

   Depositor

   Charles Miller

   Article

   Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

   Insert

   AR (AR Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pAG426GAL-EGFP

    Plasmid

    #166143

    Purpose

    EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG426GAL-mRuby3

    Plasmid

    #166144

    Purpose

    mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LT2_14_GlyOx

    Plasmid

    #177711

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS315e_pGal-dCas9-PmCDA1

    Plasmid

    #79615

    Purpose

    Expresses dCas9-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROS10

    Plasmid

    #107924

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pV1393

    Plasmid

    #111430

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNB768

    Plasmid

    #60139

    Purpose

    Green click beetle luciferase from Promega driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBG99
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZJOM44

    Plasmid

    #133653

    Purpose

    Integration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SNF7-mCherry (SNF7 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pATP403red-hENT1

    Plasmid

    #166027

    Purpose

    Expresses human equilibrative nucleoside transporter 1 (hENT1)

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    human equilibrative nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTDH3

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p416 25Q GAL

    Plasmid

    #1185

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYES2.1-Ago1

    Plasmid

    #22306

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Oct 23;326(5952):544-550.

    Insert

    AGO1
    

    Use

    2micron

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge