8,404 results
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Plasmid#107926Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEL13
Plasmid#107919Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH847-gst-optsc
Plasmid#96908PurposePlasmid for yeast expression of GST encoded by preferred codonsDepositorArticleInsertyeast codon optimised glutathione-S transferase
Tags8xhistidineExpressionYeastPromoterTDH3Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mEGFP-kanMX6
Plasmid#105146PurposeYeast gene targetingDepositorInsertmEGFP-kanMX6
TagsmEGFPExpressionBacterial and YeastMutationA206KAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES841
Plasmid#89194PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
ExpressionYeastPromoterpZAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN3FS-CYC1
Plasmid#85783PurposeN-ICE plasmid pH3FS-CYC1DepositorInsertloxP-NatMX-loxP-CYC1p-3FLAG
UseCre/LoxExpressionYeastMutation*(see below)Available SinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH-HHT2
Plasmid#86482Purposeyeast expression of HHT2 with His10-TEV-HA tagDepositorAvailable SinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEGFP-FKBP(M)x4
Plasmid#85004PurposeExpresses mEGFP-FKBP(M)x4DepositorInsertEGFP-FKBP(M)x4
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(L,V)
Plasmid#85000PurposeExpresses E2-Crimson-FKBP(L,V) in yeastDepositorInsertE2-Crimson-FKBP(LV)
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3050(gRNA XII-5)
Plasmid#73292PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2909(XII-5 MarkerFree)
Plasmid#73281PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3020(gRNA X-2)
Plasmid#73282PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNXRVb-HACre
Plasmid#78401Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
hygMX6
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-zeoCV
Plasmid#73890Purposefission yeast drug selection markerDepositorInsertzeoCV
ExpressionBacterial, Mammalian, and Y…PromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLM
Plasmid#64168Purposeharbours marker genes HIS3, LEU2 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV124
Plasmid#63190PurposepRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2793
Plasmid#64043PurposepCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoter and noneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r10
UseCRISPR and Synthetic BiologyExpressionYeastPromoterADH1Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD6-pGRR-r11-r10-yeGFP
Plasmid#74368PurposepGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.DepositorArticleInsertyeGFP
UseCRISPR and Synthetic BiologyExpressionYeastPromoterpGRR-r11-r10Available SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only