8,057 results

1. p404GAL1

   Plasmid

   #17417

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GAL1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

   Plasmid

   #24549

   Depositor

   Thomas Wandless

   Article

   Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

   Insert

   pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   1. Truncated version of ADH700 promoter (SbfI/Spe…

   Promoter

   Available since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJZC533

   Plasmid

   #135435

   Purpose

   Yeast integrating plasmid for pTEF1-mCherry reporter gene

   Depositor

   Jesse Zalatan

   Article

   Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   pTEF1

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENO1-iRFP-NATr

   Plasmid

   #129731

   Purpose

   Integration plasmid to overexpress iRFP in Candida albicans

   Depositor

   Robert Wheeler

   Article

   Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

   Insert

   iRFP-Candida albicans codon-optimized
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ENO1 from Candida albicans

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ106: pRS426-His-MBP-NlovFz2_Native

   Plasmid

   #205253

   Purpose

   S. cerevisiae expression vector for NlovFz2 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   NlovFz2
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBP-2_6.sensor

   Plasmid

   #162800

   Purpose

   Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   2_6_RNA-device
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p415TEF cyto roGFP2-Grx1(yeast)

   Plasmid

   #65004

   Purpose

   Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

   Insert

   Glutaredoxin-1
   

   Use

   Tags

   roGFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBD-pZ-FLAG

   Plasmid

   #183148

   Purpose

   Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Insert

   GAL4 BD
   

   Use

   Yeast two-hybrid

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTUP1

   Plasmid

   #104341

   Purpose

   Express TUP1

   Depositor

   Alexander Varshavsky

   Article

   Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

   Insert

   TUP1 (TUP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG306GPD-ccdB-DsRed

    Plasmid

    #14356

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJ45: pRS426-His-MBP-SpuFz1_Native

    Plasmid

    #205252

    Purpose

    S. cerevisiae expression vector for SpuFz1 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEZY45

    Plasmid

    #18704

    Purpose

    pJG4-5 converted Gateway Destination vector for yeast two-hybrid prey vector

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Two-hybrid prey vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gal1/10p CAS9 Gal1/10p I-SceI

    Plasmid

    #182519

    Purpose

    Galactose-Induced Expression vector for Cas9 and I-SceI.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Inserts

    I-SceI
    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB2794

    Plasmid

    #63651

    Purpose

    pCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPpT4- PGK1prom-TIR1-FLAG

    Plasmid

    #189724

    Purpose

    Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMJA103

    Plasmid

    #128519

    Purpose

    Expresses human amyloid Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme D67H (LYZ Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. DdMCU

    Plasmid

    #67642

    Purpose

    DdMCU-Flag in pACT2 vector human codon optimised

    Depositor

    Vamsi Mootha

    Article

    Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    DdMCU
    

    Use

    Tags

    Flag tag with linker

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSP-G2

    Plasmid

    #64737

    Purpose

    Yeast expression plasmid

    Depositor

    Jens Nielsen

    Article

    Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACT2-μ3A

    Plasmid

    #198174

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitution in codon 225

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACT2-μ3B

    Plasmid

    #198175

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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