Skip to main content
Addgene

8,030 results

Showing: 3201 - 3240 of 8030 results
  1. pGBW-m4046249

    Plasmid
    #148991
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pGBW-m4046230

    Plasmid
    #145454
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. ymScarletI-YPET pDRF1-GW

    Plasmid
    #168063
    Purpose
    FRET fusion between ymScarletI and YPET for use in yeast
    Depositor
    Insert
    ymScarletI-YPET
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pGBW-m4046658

    Plasmid
    #149226
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp10
    Insert
    SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pGBW-m4046682

    Plasmid
    #149197
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pGBW-m4046642

    Plasmid
    #149164
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp8
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pGBW-m4046687

    Plasmid
    #149148
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp7
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pGBW-m4046268

    Plasmid
    #145423
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. pCfB4782

    Plasmid
    #106141
    Purpose
    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pCfB6681

    Plasmid
    #106157
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pGBW-m4046216

    Plasmid
    #145490
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pJK371

    Plasmid
    #73596
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
    Depositor
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Point mutants in FAD2-3 ORF relative to deposited…
    Promoter
    GAL1 and GAL10
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pGBW-m4046286

    Plasmid
    #149147
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Insert
    SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  14. pJT2

    Plasmid
    #98306
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Insert
    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    ERG20 N127W
    Promoter
    Available since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. ymScarletI-ymNeongreen pDRF1-GW

    Plasmid
    #168067
    Purpose
    FRET fusion between ymScarletI and ymNeongreen for use in yeast
    Depositor
    Insert
    ymScarletI-ymNeongreen
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pTIN-ura4

    Plasmid
    #161808
    Purpose
    A ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pho1
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  17. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. UASgal-A9-Pleum

    Plasmid
    #60343
    Purpose
    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
    Depositor
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  19. pCHKU38.2-2.2

    Plasmid
    #110670
    Purpose
    Expression of a fungal biosynthetic gene cluster
    Depositor
    Insert
    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. GTL-g

    Plasmid
    #81099
    Purpose
    Gene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 3201 - 3240 of 8030 results