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pGBW-m4046442
Plasmid#145550PurposeYeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pXRU3
Plasmid#63152PurposeUra3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE MET17
Plasmid#122880PurposeMET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Pfba1-PYC-Trpl15a
Plasmid#127738PurposeYeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available sinceJan. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK273
Plasmid#71563PurposeProduces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeastDepositorInsertdelta12 fatty acid desaturase (form 2)
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-DsRed
Plasmid#14365DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mm
Plasmid#51059PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
LHP585 - Ub-CYC1
Plasmid#32168DepositorUseTagsExpressionYeastMutationPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
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Gal tr-lux
Plasmid#1330DepositorInsertBacterial luciferase-V.h.CTP5
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-mYFP
Plasmid#105141PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mYFP
UseTags41nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter41nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNC891
Plasmid#20397DepositorInsertYFP-reporter
UseTagsExpressionYeastMutationX=M (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCfB3053(gRNA X-2, XI-5, XII-4)
Plasmid#73295PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVG46
Plasmid#164704PurposeyEVenus under the control of PHO5 promoterDepositorInsertPHO5pr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXRH3
Plasmid#63145PurposeHis3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYEXC3H
Plasmid#49026PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionYeastMutationPromoterGAL1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-EYFP-ccdB
Plasmid#14341DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KEX2-iGFPx6
Plasmid#105264PurposePlasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.DepositorInsertKex2 portion (KEX2 )
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
MCA2-eGFP
Plasmid#117098Purposeprotein expression in yeastDepositorInsertMCA2-eGFP
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
MCA1-eGFP
Plasmid#117097Purposeprotein expression in yeastDepositorInsertMCA1-eGFP
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F13
Plasmid#23056DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
GTT-r
Plasmid#81111PurposeGene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
LD221
Plasmid#69189PurposeIntegration of 2GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg21-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGPDluxAB(Ura)
Plasmid#1119DepositorInsertbacterial luciferase
UseTagsExpressionYeastMutationn/aPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE GFP
Plasmid#32120DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303PromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCfB8382
Plasmid#126916PurposePlasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtIDepositorInsertXdCrtI
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRM102
Plasmid#44525DepositorInsertP(GAL10)-HHT2 P(GAL1)-HHF2
UseTagsExpressionYeastMutationPromoterGAL 10Available sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2400
Plasmid#67552PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS316Nab3
Plasmid#110533PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYEXNHG3
Plasmid#49025PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastMutationPromoterGAL1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
POM43-mCitrine
Plasmid#69855PurposeN-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmCitrine minus start and stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEP1-H-Hsp82
Plasmid#1277DepositorInserthis-Hsp82 (HSP82 Budding Yeast)
UseTagsHistidinex6ExpressionYeastMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pXRkMX
Plasmid#63147PurposeKan marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS255
Plasmid#34970DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationT406A, T407APromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only
