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pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid

#73173

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Expression

Mammalian and Yeast

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

Plasmid

#72234

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

ADE3p

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS315e_pGal-PmCDA1

Plasmid

#79612

Purpose

Expresses PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Publication

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

PmCDA1

Expression

Yeast

Promoter

pGal1

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2194

Plasmid

#67545

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2195

Plasmid

#67548

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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LD231

Plasmid

#69193

Purpose

Integration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg31-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD220

Plasmid

#69188

Purpose

Integration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg20-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD217

Plasmid

#69186

Purpose

Integration of 2GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg17-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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LD206

Plasmid

#69181

Purpose

Integration of 2GFP tag at Atg6 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg6-2EGFP

Tags

2xEGFP

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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BS-His3

Plasmid

#69197

Purpose

complementation of yeast auxotrophic marker HIS3

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

HIS3

Expression

Yeast

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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FRP1432

Plasmid

#64509

Purpose

Expresses destabilized Citrine in yeast.

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

Citrine-ADH1-tail

Expression

Yeast

Promoter

TDH3

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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FRP1454

Plasmid

#64510

Purpose

Expresses destabilized Citrine in yeast.

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

Citrine-ADH1-tail

Expression

Yeast

Promoter

TDH3

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRG213MX

Plasmid

#64522

Purpose

Centromeric E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRBUBR1

Plasmid

#20198

Depositor

Alexander Varshavsky

Publication

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 (UBR1 Budding Yeast)

Tags

HA

Expression

Yeast

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Publication

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC625

Plasmid

#62321

Purpose

sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA +1x MS2, Pol II promoter with ribozyme cleavage

Expression

Yeast

Promoter

pAdh

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRPR1_c3gRNA_RPR1t

Plasmid

#64381

Purpose

encodes c3 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c3 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pYC44

Plasmid

#63903

Purpose

Initial Integrative Vector

Depositor

Irene Castano Navarro

Publication

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FRT::3'UTRcta::NAT::FRT

Expression

Yeast

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKT-SCFP3A-HIS

Plasmid

#64686

Purpose

pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

Depositor

Erin O'Shea

Publication

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

SCFP3A

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYC50

Plasmid

#63906

Purpose

Recipient integrative vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Publication

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

MYC

Expression

Yeast

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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