8,058 results

1. pAG416GAL-EMCV_3C

   Plasmid

   #201941

   Purpose

   Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   EMCV 3C protease (EMCVgp1 )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

   Plasmid

   #173453

   Purpose

   Contains mCherry-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mCherry-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREF-GFP kan

   Plasmid

   #122061

   Purpose

   reporter plasmid for termination and terminator override to express GFP. Marked with URA3

   Depositor

   Daniel Reines

   Article

   Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

   Inserts

   intergenic terminator from yeast IMD2
   green fluorescent protein
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL1 comes in pYES2

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY2

   Plasmid

   #218115

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY3

   Plasmid

   #218116

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY3-LYS-IV

   Plasmid

   #218119

   Purpose

   LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIplac211-VPS8-mCherry2Bx6

   Plasmid

   #105273

   Purpose

   Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Vps8 portion (VPS8 Budding Yeast)
   

   Use

   Tags

   mCherry2Bx6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPTK020-7-attB

   Plasmid

   #84989

   Purpose

   Type 7, BxbI recognition site

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   BxbI recognition site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   BsaI site removed (1711 t to a)

   Promoter

   Non

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIM700

   Plasmid

   #74639

   Purpose

   alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   yTagRFP-T
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. YIPlac204TC-NLS-DsRed-Express2

    Plasmid

    #25443

    Depositor

    Benjamin Glick

    Article

    Strack et al Nat Methods. 2008 Nov . 5(11):955-7.

    Insert

    NLS-DsRed-Express2
    

    Use

    Tags

    NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. YIp128-CUP1-NUbo-GFP-His6

    Plasmid

    #212790

    Purpose

    Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-GFP-His6
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKar2-msGFP-HDEL

    Plasmid

    #117668

    Purpose

    Localisation of Endoplasmic Reticulum

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pKar2-msGFP-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains the Kar2 promoter and signal sequence fr…

    Promoter

    pKar2

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPICZac-STE2-1D4

    Plasmid

    #133437

    Purpose

    Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S/C252A

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS303-LacZ

    Plasmid

    #1169

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    LACZ
    

    Use

    Tags

    GAL1:10 promoter

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

    Plasmid

    #178989

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLH_Scr16

    Plasmid

    #100538

    Purpose

    CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

    Inserts

    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Cdc1 NLS, CreC, CreN, and SV40 NLS

    Expression

    Yeast

    Mutation

    AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A

    Promoter

    FBA1 and TDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV115

    Plasmid

    #63182

    Purpose

    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. p41Hyg 1-F GW

    Plasmid

    #58547

    Purpose

    CEN vector with Hygromycin resistance marker for single Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMAL498

    Plasmid

    #169154

    Purpose

    Encodes OptoQ-INVRT4

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNB648

    Plasmid

    #60140

    Purpose

    NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    NLuc
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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