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Plasmid#103875PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianusDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mNeonGreen-KanMX6
Plasmid#129099PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC-humanADAR1
Plasmid#198235PurposeExpresses human ADAR1 in yeastDepositorInserthuman ADAR1 (ADAR Human)
UseTagsHis10_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA Ser promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::HIS3
Plasmid#227596PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::HIS3
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188
Plasmid#67296PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
p426*-SaSgH
Plasmid#136994PurposeHelper plasmid for SaCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSB-HYG
Plasmid#166699PurposeCombined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ155
Plasmid#98411PurposePombe Expression Vector - nmt1 promoter with Sp.ura4 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
SZ_EC_02
Plasmid#155341PurposeOverexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XRDepositorInsertstef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator
UseEasycloneyali insert vectorTagsExpressionYeastMutationPromotergapdh, pyk1, and tef1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only