8,173 results
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Plasmid#235757PurposeExpression of FL ScVPS34-3xFLAG under its own promoterDepositorInsertVPS34
Tags3xFLAGExpressionYeastPromoterScVPS34 promoterAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO760
Plasmid#235759PurposeExpression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoterDepositorInsertVPS38 delta C2
Tags3xFLAGExpressionYeastMutationaa 1-154 deletedPromoterScVPS38 promoterAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO761
Plasmid#235760PurposeExpression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoterDepositorInsertVSP38 delta BARA2
Tags3xFLAGExpressionYeastMutationaa 320-440 deletedPromoterScVPS38 promoterAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO764
Plasmid#235761PurposeExpression of ScVPS38-EGFP under its own promoterDepositorInsertVPS38
TagsEGFPExpressionYeastPromoterScVPS38 promoterAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO765
Plasmid#235762PurposeExpression of ScVPS38 delta C2 (1-154) -EGFP under its own promoterDepositorInsertVPS38
TagsEGFPExpressionYeastMutationaa 1-154 deletedPromoterScVPS38 promoterAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO766
Plasmid#235763PurposeExpression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoterDepositorInsertVSP38 delta BARA2
TagsEGFPExpressionYeastMutationaa 320-440 deletedPromoterScVPS38 promoterAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO932
Plasmid#235764PurposeExpression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoterDepositorInsertVPS34 HELCAT
Tags3xFLAGExpressionYeastMutationaa 268-875PromoterScVPS34 promoterAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO933
Plasmid#235765PurposeExpression of ScVPS34 delta C154-V202-3xFLAG under its own promoterDepositorInsertVPS34 delta C154-V202
Tags3xFLAGExpressionYeastMutationaa 154-202 deletedPromoterScVPS34 promoterAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO503
Plasmid#235743PurposeProtein expression of KmTor by integrating ZZ-KmTOR into the K. marxianus genomeDepositorInsertKmTOR
TagsZZ-3xTEVExpressionYeastMutationaa 4-4609Available SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO524
Plasmid#235744PurposeProtein expression of KmLst8 by integrating KmLST8 into the K. marxianus genomeDepositorInsertKmLST8
ExpressionYeastAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-RWT4Ca_Reg1
Plasmid#237525PurposeYeast two-hybridDepositorInsertRwt4Ca_Reg1
ExpressionYeastMutationRemoved binding region 1Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-RWT4Ca+C-Tail
Plasmid#237526PurposeYeast two-hybridDepositorInsertRwt4Ca+C-Tail
ExpressionYeastMutationAdd C-terminal extensionAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-RWT4Ca
Plasmid#237524PurposeYeast two-hybridDepositorInsertRwt4Ca
ExpressionYeastAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-AvrPWT4
Plasmid#237527PurposeYeast two-hybridDepositorInsertAvrPwt4
ExpressionYeastAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-VirPWT4
Plasmid#237528PurposeYeast two-hybridDepositorInsertVirPwt4
ExpressionYeastAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-NET1
Plasmid#232893PurposePlasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.DepositorAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-KAE1
Plasmid#232891PurposePlasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.DepositorAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-VRG4
Plasmid#232899PurposePlasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.DepositorAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-SAM50
Plasmid#232895PurposePlasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.DepositorAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL-mGold2s
Plasmid#231761PurposeExpression of mGold2s in yeast cellsDepositorInsertmGold2s
ExpressionYeastMutationmGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterpTDH(GAP promoter)Available SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-SoxN-A22-EGFP
Plasmid#181731PurposeGalactose inducible expression of SoxN-A22-EGFPDepositorInsertSoxN-A22-EGFP (SoxN Fly)
Tags3xFLAG-EGFPExpressionYeastMutationextended repeat of 22 Ala residues replacing nati…Available SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
P415 ymNeongreen
Plasmid#224110PurposeExpressing ymNeongreen in yeastDepositorInsertymNeongreen
ExpressionYeastAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-Hyg
Plasmid#236486PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.DepositorInsertmStayGold
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-Nat
Plasmid#236485PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.DepositorInsertmStayGold
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-1
Plasmid#236472PurposePlasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.DepositorInsertspH2Ap-3xmCherry spH2Bp-3xGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-3
Plasmid#236474PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApACT1 promoter.DepositorInsertscACT1p-3xmCherry apACT1p-3xGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyS
Plasmid#181728PurposeGalactose inducible expression of 3xFLAG-PolySDepositorInsert3xFLAG-PolyS
Tags3xFLAGExpressionYeastAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyA
Plasmid#181726PurposeGalactose inducible expression of 3xFLAG-PolyADepositorInsert3xFLAG-PolyA
Tags3xFLAGExpressionYeastAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyL
Plasmid#181727PurposeGalactose inducible expression of 3xFLAG-PolyLDepositorInsert3xFLAG-PolyL
Tags3xFLAGExpressionYeastAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyC
Plasmid#181729PurposeGalactose inducible expression of 3xFLAG-PolyCDepositorInsert3xFLAG-PolyC
Tags3xFLAGExpressionYeastAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-4
Plasmid#236475PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApTUB1 promoter.DepositorInsertscACT1p-3xmCherry apTUB1p-3xGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-2
Plasmid#236473PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.DepositorInsertspH2Bp-3xmCherry scACT1p-3xGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-G418
Plasmid#236487PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on G418.DepositorInsertmStayGold
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-Nat
Plasmid#236488PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.DepositorInsertmCherry
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-Hyg
Plasmid#236480PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Hyg.DepositorInsertsfGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-G418
Plasmid#236490PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.DepositorInsertmCherry
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-Nat
Plasmid#236491PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.DepositorInsertmScarlet
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-Hyg
Plasmid#236489PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.DepositorInsertmCherry
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-G418
Plasmid#236496PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.DepositorInsertDendra2
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-Nat
Plasmid#236476PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.DepositorInsertGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-Hyg
Plasmid#236495PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Hyg.DepositorInsertDendra2
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-Hyg
Plasmid#236483PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.DepositorInsertmNeonGreen
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-G418
Plasmid#236493PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.DepositorInsertmScarlet
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-Hyg
Plasmid#236492PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.DepositorInsertmScarlet
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-Hyg
Plasmid#236477PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.DepositorInsertGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-Nat
Plasmid#236479PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.DepositorInsertsfGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-Nat
Plasmid#236482PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.DepositorInsertmNeonGreen
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-G418
Plasmid#236478PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.DepositorInsertGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 9bp dist
Plasmid#234131PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 12bp dist
Plasmid#234130PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only