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Yeast Expression

7,935 results

Showing: 4601 - 4650 of 7935 results

  1. pdnCas3-APOBEC1

    Plasmid
    #229537
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229537-plasmid-map-sequence-id-459005

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  2. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

    Enlarge

  3. pCas3-CDA1

    Plasmid
    #229534
    Purpose
    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229534-plasmid-map-sequence-id-458891

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  4. pCfb13199

    Plasmid
    #219874
    Purpose
    The base plasmid of TUNEYALI forTF09
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219874-plasmid-map-sequence-id-472125

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  5. pCfb13218

    Plasmid
    #219893
    Purpose
    The base plasmid of TUNEYALI for TF28
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219893-plasmid-map-sequence-id-458635

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  11. pVG134

    Plasmid
    #227770
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Glu84Gly) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Glu84Gly mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227770-plasmid-map-sequence-id-458112

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  12. pVG178

    Plasmid
    #227761
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Glu84KLys mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227761-plasmid-map-sequence-id-458111

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  13. pVG144

    Plasmid
    #227760
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Val121Met mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227760-plasmid-map-sequence-id-458109

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  14. pVG150

    Plasmid
    #227759
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Ser137Asn mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227759-plasmid-map-sequence-id-458108

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  15. pVG124

    Plasmid
    #227758
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Thr83Ser mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227758-plasmid-map-sequence-id-458107

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  16. pVG121

    Plasmid
    #227757
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Gly80Glu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227757-plasmid-map-sequence-id-458105

    Enlarge

  17. pVG123

    Plasmid
    #227755
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Ala157Val mutant
    Promoter
    PGK1pr
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    227755-plasmid-map-sequence-id-458101

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  18. pVG143

    Plasmid
    #227754
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Leu87Ile
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227754-plasmid-map-sequence-id-458037

    Enlarge

  19. pVG147

    Plasmid
    #227753
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Gly94Cys mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227753-plasmid-map-sequence-id-458034

    Enlarge

  20. pVG120

    Plasmid
    #227752
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Asp89Tyr mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227752-plasmid-map-sequence-id-458027

    Enlarge

  21. pVG137

    Plasmid
    #227751
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Val201Leu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227751-plasmid-map-sequence-id-458026

    Enlarge

  22. pVG136

    Plasmid
    #227750
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Pro165Ser mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227750-plasmid-map-sequence-id-458025

    Enlarge

  23. pVG135

    Plasmid
    #227749
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Lys99Glu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227749-plasmid-map-sequence-id-458022

    Enlarge

  24. pVG119

    Plasmid
    #227748
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Val139Leu mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227748-plasmid-map-sequence-id-458021

    Enlarge

  25. pVG118

    Plasmid
    #227747
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Asp18Tyr) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Asp18Tyr mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227747-plasmid-map-sequence-id-458020

    Enlarge

  26. pVG117

    Plasmid
    #227746
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Asp18Tyr, Val139Leu) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Asp18Tyr, Val139Leu double mutant
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227746-plasmid-map-sequence-id-458019

    Enlarge

  31. pOGS539_haPD-1

    Plasmid
    #226716
    Purpose
    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)
    Depositor
    Gautam Dantas
    Article
    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2
    Insert
    High affinity PD-1 microbody
    Use
    Tags
    HA tag and Mating factor alpha secretion signal
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226716-plasmid-map-sequence-id-455452

    Enlarge

  32. pOGS539_rPD-L1nb

    Plasmid
    #226717
    Purpose
    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)
    Depositor
    Gautam Dantas
    Article
    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2
    Insert
    Recombinant PD-L1 nanobody
    Use
    Tags
    HA tag, Histidine tag, and Mating factor alpha se…
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226717-plasmid-map-sequence-id-455449

    Enlarge

  40. pCfb13239

    Plasmid
    #219912
    Purpose
    The base plasmid of TUNEYALI for TF49
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219912-plasmid-map-sequence-id-456119

    Enlarge

  44. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

    Plasmid
    #227608
    Purpose
    Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    aa 2-83 deleted
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227608-plasmid-map-sequence-id-455573

    Enlarge

  45. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

    Plasmid
    #227609
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-544, 651-757 only [TM deleted]
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227609-plasmid-map-sequence-id-455572

    Enlarge

  47. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid
    #227611
    Purpose
    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    MBP, 10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-88 only
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227611-plasmid-map-sequence-id-455570

    Enlarge

  48. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid
    #227610
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-400, 706-757 [TM and HB2 deleted]
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227610-plasmid-map-sequence-id-455569

    Enlarge

Showing: 4601 - 4650 of 7935 results