8,043 results

1. LHP599 - pACT2-Ub

   Plasmid

   #32170

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Gal4 AD and HA

   Expression

   Yeast

   Mutation

   Promoter

   pADH1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pPS809

   Plasmid

   #8935

   Depositor

   Pamela Silver

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pXP120

   Plasmid

   #26826

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDSM-ab-Psptef1-NAT-Ttip1

   Plasmid

   #127747

   Purpose

   Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   nourseothricin N-acetyltransferase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Psptef1

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJLI-SUP35 (delta)2-5

   Plasmid

   #1138

   Depositor

   Susan Lindquist

   Article

   Liu et al Nature 1999 Aug 5;400(6744):573-6.

   Insert

   sup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   last four repeats of sup35 N domain deleted, but …

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDRF1-GW mkok-T2A-mTurquoise2

   Plasmid

   #118428

   Purpose

   Produces equimolar expression of mKokappa and mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   mkok-T2A-mTq2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMEG_BB3_YL18H_AB

   Plasmid

   #117827

   Purpose

   GoldenMOCS backbone for 1 TU in Y. lipolytica

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMEG_BB3_YL18H_AC

   Plasmid

   #117828

   Purpose

   GoldenMOCS backbone for 2 TU in Y. lipolytica

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMEG_BB3_YL68N_AE

   Plasmid

   #117832

   Purpose

   GoldenMOCS backbone for 4 TU in Y. lipolytica

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMEG_BB3_YL68N_AC

    Plasmid

    #117830

    Purpose

    GoldenMOCS backbone for 2 TU in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMEG_BB3_YL68N_AB

    Plasmid

    #117829

    Purpose

    GoldenMOCS backbone for 1 TU in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMEG_BB3_YL68N_AD

    Plasmid

    #117831

    Purpose

    GoldenMOCS backbone for 3 TU in Y. lipolytica

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPTK012-3a-Inulinase-αMFΔ

    Plasmid

    #84971

    Purpose

    Type 3a, Inulinase followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Inulinase followed by αMFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPTK004-2-pTPI1

    Plasmid

    #84963

    Purpose

    Type 2, pTPI1, Triose phosphate isomerase 1 promoter

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Triose phosphate isomerase 1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-6xGLY-HSV-HIS3MX6

    Plasmid

    #20774

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS415-NAR1

    Plasmid

    #10995

    Depositor

    Francisco Malagon

    Insert

    NAR1 (NAR1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YIp LYS2-HphMx

    Plasmid

    #122919

    Purpose

    LYS2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIp LYS2-NatMx

    Plasmid

    #122921

    Purpose

    LYS2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV10.F6.loxP

    Plasmid

    #63201

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCHKU34.1-2.2

    Plasmid

    #115534

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    PKS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYC66

    Plasmid

    #63929

    Purpose

    Recipient replicative vector to construct MYC tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    MYC

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. YIplac204-T|C-iGFP-RCY1

    Plasmid

    #105281

    Purpose

    Overexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Rcy1 (RCY1 Budding Yeast)
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Ube2e2

    Plasmid

    #31414

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2E2 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

24. Ube2e1

    Plasmid

    #31413

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2E1 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    *see comments

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pAG415GPD-EYFP-ccdB

    Plasmid

    #14338

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCfB4613

    Plasmid

    #126752

    Purpose

    Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker

    Depositor

    Michael Jensen

    Article

    Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

    Insert

    CDC14 (CDC14 Budding Yeast)
    

    Use

    Integrative plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTRX2_5xUAS

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAG423GPD-EYFP-ccdB

    Plasmid

    #14342

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 7860 pRS305-pUG72-HSC82in

    Plasmid

    #10898

    Depositor

    Susan Lindquist

    Article

    Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

    Insert

    HSC82 URA3 (HSC82 Budding Yeast)
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pESC/CURT_fluoB

    Plasmid

    #117997

    Purpose

    Expression of CURT_fluoB controlled by the Gal1 promoter

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    CURT_fluoB
    

    Use

    Yeast/bacteria binary vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAS404

    Plasmid

    #15978

    Depositor

    Eishi Noguchi

    Article

    Nakashima et al Genetics. 1999 Jul . 152(3):853-67.

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJW1671

    Plasmid

    #112045

    Purpose

    CEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    ORM1
    

    Use

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCfB3037(XI-5 MarkerFree)

    Plasmid

    #73277

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. GAL-CDC12(WT)

    Plasmid

    #1165

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    CDC12 (CDC12 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GAL-CDC12(K351N)

    Plasmid

    #1166

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    CDC12 (CDC12 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K351N

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CDC12(E368Q)

    Plasmid

    #1167

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    CDC12 (CDC12 Budding Yeast)
    

    Use

    Tags

    266bp of upstream sequence

    Expression

    Yeast

    Mutation

    E368Q

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p416TEF-NQYFP

    Plasmid

    #99552

    Purpose

    Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

    Depositor

    Liming Li

    Article

    Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

    Insert

    Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAG425GAL-ccdB-TAP

    Plasmid

    #14273

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LHP1263 - HA-VPS9

    Plasmid

    #32431

    Depositor

    Linda Hicke

    Article

    Shih et al EMBO J. 2003 Mar 17;22(6):1273-81.

    Insert

    VPS9 (VPS9 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    Promoter

    Lac

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

39. UASGAL-Pleum

    Plasmid

    #60333

    Purpose

    Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pPS2038

    Plasmid

    #1371

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    SSA4-U1A-ASH1 3' UTR (SSA4 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only