8,418 results
-
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
ExpressionYeastAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLK88 - Edit-directing plasmid base
Plasmid#98814PurposeEdit-directing plasmid baseDepositorInsertCAN1.y gRNA
UseCRISPRExpressionYeastAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*
Plasmid#89071Purposegenomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-PYK1
Plasmid#85781PurposeN-ICE plasmid pH3FS-PYK1DepositorInsertloxP-HphMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-DsRed (JBEI-15461)
Plasmid#87935PurposeCloning vector for co-expressing a gene with 4CL5 in S.cerevisiaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK020-7-attB
Plasmid#84989PurposeType 7, BxbI recognition siteDepositorInsertBxbI recognition site
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1711 t to a)PromoterNonAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
p426TEF-LptTPS-A
Plasmid#89468PurposeExpression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene productionDepositorInsertLptTPS-A
TagsnoneExpressionYeastPromoterpBR322 origin of replication for propagation in E…Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK009-3a-αAmylase-αMFΔ
Plasmid#84968PurposeType 3a, α-Amylase followed by αMFΔ - secretion signalDepositorInsertα-Amylase followed by MFΔ secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK018-3b-RFP_Sec
Plasmid#84977PurposeType 3b, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pK3F
Plasmid#85770PurposeN-ICE plasmid pK3FDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pILGFPH7
Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
ExpressionYeastPromoterADH2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyExpressionBacterial and YeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3039(XII-2 MarkerFree)
Plasmid#73279PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3046(gRNA XI-5)
Plasmid#73288PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3048(gRNA XII-2)
Plasmid#73290PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTH-ir
Plasmid#81107PurposeGene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNXRVc-HACre
Plasmid#78400Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
natMX6
ExpressionYeastAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FTKnatAX
Plasmid#73899Purposefission yeast drug counter-selectable selection markerDepositorInsertTK-nat
ExpressionYeastAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only