7,935 results

1. pNK7182

   Plasmid

   #219767

   Purpose

   Vector for expression PpASCL in yeast (P.pastoris)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PpASCL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNK7188

   Plasmid

   #219768

   Purpose

   Vector for expression Pv4CL1 in yeast (P.pastoris)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   Pv4CL1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfb13220

   Plasmid

   #219895

   Purpose

   The base plasmid of TUNEYALI for TF30

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDRF1-GW mKelly-T2A-mtq2

   Plasmid

   #205506

   Purpose

   Expression of mKelly-T2A-mtq2 in yeast

   Depositor

   Bas Teusink

   Insert

   mKelly-T2A-mtq2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG426-GAL-APP-CTF

   Plasmid

   #181732

   Purpose

   Galactose inducible expression of APP-CTF

   Depositor

   Alejandro Chavez

   Insert

   APP-CTF (APP Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C-terminal fragment (aa 671-770)

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWR95(URA3)

   Plasmid

   #203917

   Purpose

   BLINCAR plasmid for gene deletion, URA3 target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   URA3 upstream homology
   URA3 downstream homology
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWR91

   Plasmid

   #203915

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   CRF1 plus flanking (CRF1 S. stipitis (yeast))
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWR90

   Plasmid

   #203914

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   GSC2 plus flanking (GSC2 S. stipitis (yeast))
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWR89

   Plasmid

   #203423

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   EGC3 plus flanking (EGC3 S. stipitis (yeast))
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWR88

    Plasmid

    #203422

    Purpose

    empty BLINCAR plasmid for payload delivery, no payload, no genomic target

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    CAR copy 1
    P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
    CAR copy 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWR63(YUM1)

    Plasmid

    #203336

    Purpose

    episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    YUM1-targeting sequence
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWR63

    Plasmid

    #203331

    Purpose

    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWR76

    Plasmid

    #202776

    Purpose

    episomal fluorescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWR23

    Plasmid

    #202770

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWR10

    Plasmid

    #202710

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWR9

    Plasmid

    #202645

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYES2/NTA/Leu-LegA7

    Plasmid

    #216518

    Purpose

    Expression of LegA7

    Depositor

    Ralph Isberg

    Insert

    Leg A7
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 WT

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYES2/NTA/Leu-LegA7Δank

    Plasmid

    #216519

    Purpose

    Expression of LegA7 ΔAnk

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 331-454

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid

    #216520

    Purpose

    Expression of LegA7 ΔAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk290
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-454

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid

    #216521

    Purpose

    Expression of LegA7 ΔNAnk264

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk264
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 264-361

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid

    #216522

    Purpose

    Expression of LegA7 ΔNAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk290
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-361

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. RT-3

    Plasmid

    #220440

    Purpose

    Base construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MV IE93 promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTEF1_MS2

    Plasmid

    #220625

    Purpose

    Source for TEF1 MS2 coat fusion protein fusion

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RT-2

    Plasmid

    #220438

    Purpose

    Base construct used to create PUP2-DADA/3HA fusion proteins in S. cerevisiae. (aka pBSII/PUP2-DADA/3HA/URA3)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RT-5

    Plasmid

    #220442

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (puf-3 Nematode)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RT-4

    Plasmid

    #220443

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (PUF3 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MAP80A_MS2

    Plasmid

    #220624

    Purpose

    tRNA reporter vector

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Leu2 2u

    Plasmid

    #220622

    Purpose

    empty backbone for shuttle vector construction

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Insert

    empty vector (LEU2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RT-1

    Plasmid

    #220434

    Purpose

    Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pReporter_ZF2_ZF1_mNeonGreen

    Plasmid

    #213834

    Purpose

    Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pReporter_ZF1_mNeonGreen_ZF2

    Plasmid

    #213835

    Purpose

    Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pReporter_ZF1_ZF2_mNeonGreen

    Plasmid

    #213836

    Purpose

    Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pReporter_ZF2_mNeonGreen_ZF1

    Plasmid

    #213837

    Purpose

    Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pReporter_mNeonGreen

    Plasmid

    #213838

    Purpose

    Facilitates genomic integration of the control reporter without zinc finger binding sites.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPtet-1_mNeonGreen_Ptet-2_mCherry

    Plasmid

    #213839

    Purpose

    Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen, mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pReporter_ZF1_mNeonGreen

    Plasmid

    #213840

    Purpose

    Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pReporter_ZF2_mNeonGreen

    Plasmid

    #213841

    Purpose

    Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPtet-2-VP16

    Plasmid

    #213842

    Purpose

    Used for expressing VP16 fused to ZF-2.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPtet-1-VP16

    Plasmid

    #213843

    Purpose

    Used for expressing VP16 fused to ZF-1.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pStorage_Ptet-1_ZF1

    Plasmid

    #213844

    Purpose

    CombiGEM storage plasmid for ZF-1 CR fusions.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pStorage_Ptet-2_ZF2

    Plasmid

    #213845

    Purpose

    CombiGEM storage plasmid for ZF-2 CR fusions.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pReporter_TetR

    Plasmid

    #213847

    Purpose

    Facilitates genomic integration of tetR expression cassette.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    TetR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCfb12622

    Plasmid

    #219929

    Purpose

    PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter ACT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCfb13237

    Plasmid

    #219910

    Purpose

    The base plasmid of TUNEYALI for TF47

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCfb12562

    Plasmid

    #219923

    Purpose

    PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter GPD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. ClhN-p500-Erg6-V5-APEX2-URA

    Plasmid

    #207070

    Purpose

    Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Erg6
    

    Use

    Tags

    V5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pERG6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA

    Plasmid

    #207064

    Purpose

    Expressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Pex3
    

    Use

    Tags

    4xV5-APEX2 and GFP

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. BS-ZJLEU-PTEF1-GFP

    Plasmid

    #207018

    Purpose

    Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. PTPI1-Dsred-URA3

    Plasmid

    #207017

    Purpose

    Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    DsRed-Express 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTPI1

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCfb13186

    Plasmid

    #219924

    Purpose

    PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter TEF
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge