8,350 results
-
Plasmid#235745PurposeProtein expression of KmKog1 in yeastDepositorInsertKmKOG1
TagsZZ-3xFLAGExpressionYeastAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO1880
Plasmid#235721PurposeExpression of FL yeast VPS15 S622D_H623D_T626E mutant -EGFP under its own promoter in yeastDepositorArticleInsertVPS15
ExpressionYeastMutationS622D, H623D, T626EPromoterScVPS15 promoterAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO634
Plasmid#235720PurposeExpression of FL yeast VPS15-EGFP under its own promoter in yeastDepositorArticleInsertVPS15
TagsEGFPExpressionYeastPromoterScVPS15 promoterAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL-mGold2s
Plasmid#231761PurposeExpression of mGold2s in yeast cellsDepositorInsertmGold2s
ExpressionYeastMutationmGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterpTDH(GAP promoter)Available SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-SoxN-A22-EGFP
Plasmid#181731PurposeGalactose inducible expression of SoxN-A22-EGFPDepositorInsertSoxN-A22-EGFP (SoxN Fly)
Tags3xFLAG-EGFPExpressionYeastMutationextended repeat of 22 Ala residues replacing nati…Available SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
P415 ymNeongreen
Plasmid#224110PurposeExpressing ymNeongreen in yeastDepositorInsertymNeongreen
ExpressionYeastAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-Hyg
Plasmid#236486PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.DepositorInsertmStayGold
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-Nat
Plasmid#236485PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.DepositorInsertmStayGold
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-1
Plasmid#236472PurposePlasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.DepositorInsertspH2Ap-3xmCherry spH2Bp-3xGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-3
Plasmid#236474PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApACT1 promoter.DepositorInsertscACT1p-3xmCherry apACT1p-3xGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyS
Plasmid#181728PurposeGalactose inducible expression of 3xFLAG-PolySDepositorInsert3xFLAG-PolyS
Tags3xFLAGExpressionYeastAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyA
Plasmid#181726PurposeGalactose inducible expression of 3xFLAG-PolyADepositorInsert3xFLAG-PolyA
Tags3xFLAGExpressionYeastAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyL
Plasmid#181727PurposeGalactose inducible expression of 3xFLAG-PolyLDepositorInsert3xFLAG-PolyL
Tags3xFLAGExpressionYeastAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyC
Plasmid#181729PurposeGalactose inducible expression of 3xFLAG-PolyCDepositorInsert3xFLAG-PolyC
Tags3xFLAGExpressionYeastAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-4
Plasmid#236475PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApTUB1 promoter.DepositorInsertscACT1p-3xmCherry apTUB1p-3xGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-2
Plasmid#236473PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.DepositorInsertspH2Bp-3xmCherry scACT1p-3xGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-G418
Plasmid#236487PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on G418.DepositorInsertmStayGold
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-Nat
Plasmid#236488PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.DepositorInsertmCherry
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-Hyg
Plasmid#236480PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Hyg.DepositorInsertsfGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-G418
Plasmid#236490PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.DepositorInsertmCherry
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-Nat
Plasmid#236491PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.DepositorInsertmScarlet
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-Hyg
Plasmid#236489PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.DepositorInsertmCherry
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-G418
Plasmid#236496PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.DepositorInsertDendra2
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-Nat
Plasmid#236476PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.DepositorInsertGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-Hyg
Plasmid#236495PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Hyg.DepositorInsertDendra2
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-Hyg
Plasmid#236483PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.DepositorInsertmNeonGreen
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-G418
Plasmid#236493PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.DepositorInsertmScarlet
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-Hyg
Plasmid#236492PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.DepositorInsertmScarlet
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-Hyg
Plasmid#236477PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.DepositorInsertGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-Nat
Plasmid#236479PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.DepositorInsertsfGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-Nat
Plasmid#236482PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.DepositorInsertmNeonGreen
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-G418
Plasmid#236478PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.DepositorInsertGFP
ExpressionYeastMutationCodon optimized for A. pullulansAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 9bp dist
Plasmid#234131PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 12bp dist
Plasmid#234130PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 27bp dist
Plasmid#234129PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 15bp dist
Plasmid#234128PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 42bp dist
Plasmid#234127PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 50bp dist
Plasmid#234112PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 46bp dist
Plasmid#234111PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6-CFAPoptim-NAT
Plasmid#221129PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMXDepositorInsertFAPoptim
ExpressionYeastAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar2
Plasmid#232739PurposeExpresses NADPH/NADP+ biosensor NAPstar2 in S. cerevisiae.DepositorInsertNAPstar2
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar4
Plasmid#232741PurposeExpresses NADPH/NADP+ biosensor NAPstar4 in S. cerevisiae.DepositorInsertNAPstar4
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar6
Plasmid#232742PurposeExpresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.DepositorInsertNAPstar6
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar7
Plasmid#232744PurposeExpresses NADPH/NADP+ biosensor NAPstar7 in S. cerevisiae.DepositorInsertNAPstar7
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-SEC61-FAPoptim
Plasmid#221126PurposeFAP-tagged marker for the ER (FAP optimized for yeast expression)DepositorInsertSEC61-FAPoptim
ExpressionYeastPromoterTEF1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC63-FAPoptim
Plasmid#221116PurposeFAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertFAPoptim-SEC63
ExpressionYeastPromoterTEF1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7_AvrSr62-1
Plasmid#233531PurposeExpresses AvrSr62-1 in yeastDepositorInsertAvrSr62-1
TagsGAL4 BD, Myc tagExpressionYeastPromoterADH1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC61-FAPoptim
Plasmid#221115PurposeFAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertSEC61-FAPoptim
ExpressionYeastPromoterTEF1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb13198
Plasmid#219873PurposeThe base plasmid of TUNEYALI forTF08DepositorInsertContains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08
ExpressionYeastAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-rHUH
Plasmid#235190PurposeTo display rHUH on yeast surfaceDepositorInsertrHUH
ExpressionYeastAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only