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9,802 results

Showing: 1041 - 1060 of 9802 results

  1. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

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  3. pET28-Nsp14-TEV-6His

    Plasmid
    #154952
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    Tags
    TEV-6His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154952-plasmid-map-sequence-id-307820

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  4. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    6His-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    154954-plasmid-map-sequence-id-307815

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  5. pENTR223 CD147, open

    Plasmid
    #149720
    Purpose
    for Gateway cloning of CD147 into destination vector
    Depositor
    Anne-Claude Gingras
    Insert
    CD147 (BSG Human)
    Use
    Gateway Cloning
    Mutation
    wt, without stop codon
    Available since
    June 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149720-plasmid-map-sequence-id-298608

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  7. pDONR207 SARS-CoV-2 M_nostop

    Plasmid
    #149322
    Purpose
    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Publication
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway Cloning
    Mutation
    Many synonymous changes due to codon optimization
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149322-plasmid-map-sequence-id-296471

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  14. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    114406-plasmid-map-sequence-id-220833

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  16. 5KO9 (HMCES)

    Plasmid
    #101877
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HMCES (HMCES Human)
    Tags
    6x His (modified C-term tag is AENLYFQSHHHHHH)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    101877-plasmid-map-sequence-id-194533

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  19. pCAGGS_HA-VP35_EBOV

    Plasmid
    #103053
    Purpose
    Mammalian expression vector encoding HA-tagged Ebola virus VP35 gene under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Publication
    Nelson et al J Virol. 2016 Jul 27;90(16):7268-84. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VP35 (VP35 Zaire ebolavirus, Mayinga)
    Tags
    HA-tag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Dec. 20, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    103053-plasmid-map-sequence-id-200886

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Showing: 1041 - 1060 of 9802 results