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9,747 results

Showing: 1041 - 1060 of 9747 results

  2. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    114406-plasmid-map-sequence-id-220833

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  4. 5KO9 (HMCES)

    Plasmid
    #101877
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HMCES (HMCES Human)
    Tags
    6x His (modified C-term tag is AENLYFQSHHHHHH)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101877-plasmid-map-sequence-id-194533

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  8. pAAV-i53-DM

    Plasmid
    #92171
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)
    Depositor
    Daniel Durocher
    Publication
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53-DM
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A, P69L, and L70V mutations and no…
    Promoter
    CMV
    Available since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92171-plasmid-map-sequence-id-182616

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  9. pMycoFos

    Plasmid
    #84577
    Purpose
    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.
    Depositor
    Nicholas Coleman
    Publication
    Ly et al J Microbiol Methods. 2011 Sep;86(3):320-
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    April 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    84577-plasmid-map-sequence-id-175425

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  10. pUS116

    Plasmid
    #84578
    Purpose
    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium.
    Depositor
    Nicholas Coleman
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Jan. 17, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    84578-plasmid-map-sequence-id-389132

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  11. InsRA-TagBFP

    Plasmid
    #79791
    Purpose
    Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Publication
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Insert
    InsRA (INSR Human)
    Tags
    TagBFP extracellular
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 5, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79791-plasmid-map-sequence-id-149402

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  13. pET28-MKH8SUMO

    Plasmid
    #79526
    Purpose
    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    8xHis-Thrombin-SUMO-TEV and TEV cleavage site
    Expression
    Bacterial
    Available since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    11 Articles

  15. pCDH-CMV4

    Plasmid
    #72284
    Purpose
    Express gene of interest under CMV promoter. WPRE has been removed to increase the cloning capacity.
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    72284-plasmid-map-sequence-id-310586

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  16. pCDH-CB-iCre

    Plasmid
    #72257
    Purpose
    Express iCre under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Available since
    Jan. 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
    72257-plasmid-map-sequence-id-308172

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  17. pCDH-EF1-FLPe

    Plasmid
    #72262
    Purpose
    Express FLPe under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    elongation factor-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    72262-plasmid-map-sequence-id-131821

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  18. mEos3.2-Vinculin-N-21

    Plasmid
    #66952
    Purpose
    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    V234L in Vinculin
    Promoter
    CMV
    Available since
    July 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. 4R3H

    Plasmid
    #64652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles

  20. mEmerald-ALK5-N-13

    Plasmid
    #62751
    Purpose
    Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    ALK5 (TGFBR1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1041 - 1060 of 9747 results