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Showing: 1 - 10 of 5439 results

  2. SPIN2B (SPIN2BA-c011)

    Plasmid
    #98238
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPIN2BA (SPIN2B Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98238-plasmid-map-sequence-id-193417

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  4. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Article
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS2-Spike_del21_D614G (S Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158762-plasmid-map-sequence-id-309495

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  5. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

    Plasmid
    #204146
    Purpose
    Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes
    Depositor
    Jesse Bloom
    Article
    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.
    Inserts
    SARS-CoV-2 BA.1 variant spike
    zsGreen-T2A-PuR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    21 amino acid cytoplasmic tail deletion
    Promoter
    CMV and TRE3G
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    204146-plasmid-map-sequence-id-404779

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  6. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Use
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  7. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

    Plasmid
    #204151
    Purpose
    Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes
    Depositor
    Jesse Bloom
    Article
    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.
    Insert
    SARS-CoV-2 Delta variant spike
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    21 amino acid cytoplasmic tail deletion
    Promoter
    TRE3G
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    204151-plasmid-map-sequence-id-404782

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  8. pTFG005_Spike_Inducible_3xFlag

    Plasmid
    #191578
    Purpose
    Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Lentiviral
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    191578-plasmid-map-sequence-id-442483

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  9. pSPIKE-P

    Plasmid
    #101172
    Purpose
    chimeric spike resembling V4 region of prokaryotic 16S rRNA to be used in metagenomics
    Depositor
    Philip Poole
    Insert
    V4 16S rRNA chimeric spike
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    101172-plasmid-map-sequence-id-194323

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  10. pSPIKE-F

    Plasmid
    #101174
    Purpose
    chimeric spike resembling fungal ITS region to be used in metagenomics
    Depositor
    Philip Poole
    Insert
    chimeric fungal ITS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    101174-plasmid-map-sequence-id-194321

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Showing: 1 - 10 of 5439 results