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Showing: 1 - 20 of 718 results

  7. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid
    #155064
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155064-plasmid-map-sequence-id-308583

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  8. pUDE735

    Plasmid
    #103024
    Purpose
    expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)
    Depositor
    Jean-Marc Daran
    Article
    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125
    Insert
    crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SNR52
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    103024-plasmid-map-sequence-id-198968

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  9. pLCHKO_TK1_HPRT1_Lb_5

    Plasmid
    #155063
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155063-plasmid-map-sequence-id-308581

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  10. pLCHKO_TK1_HPRT1_Lb_4

    Plasmid
    #155062
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155062-plasmid-map-sequence-id-308576

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  11. pCR-Hyg

    Plasmid
    #158708
    Purpose
    For cloning and constitutive expression of crRNAs in mycobacterium
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    crRNA
    Use
    CRISPR and Synthetic Biology; Mycobacteria expres…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Hsp60
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158708-plasmid-map-sequence-id-320872

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  12. pCR-Zeo

    Plasmid
    #158709
    Purpose
    For cloning and constitutive expression of crRNAs in mycobacterium
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    crRNA
    Use
    CRISPR and Synthetic Biology; Mycobacteria expres…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Hsp60
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158709-plasmid-map-sequence-id-320874

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  13. pLCHKO_TK1_HPRT1_Lb_3

    Plasmid
    #155061
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155061-plasmid-map-sequence-id-308580

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  16. pCoHygro L16_shRNA of spliced Copia

    Plasmid
    #71389
    Purpose
    knock down expression of spliced Copia in Drosophila melanogaster
    Depositor
    Timothy Nilsen
    Insert
    Copia
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6
    Available since
    Nov. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    71389-plasmid-map-sequence-id-129772

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  17. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

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  18. pX459-puro-hARAF-1

    Plasmid
    #185367
    Purpose
    For mammalian expression of guide RNA: caccgTGGTCTACCGACTCATCAAG that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185367-plasmid-map-sequence-id-363617

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  20. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    James Collins
    Article
    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182746-plasmid-map-sequence-id-358079

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Showing: 1 - 20 of 718 results