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Plasmid#192380PurposeTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2
Plasmid#11343DepositorTypeEmpty backboneUseTagsGal4 Activation DomainExpressionYeastMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2gtwy
Plasmid#11346DepositorInsertgateway cassette
UseTagsGal4 Activation DomainExpressionYeastMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ4
Plasmid#198176PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 μ4
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ2
Plasmid#198173PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-2 μ2
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ
Plasmid#192381PurposeTo test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ
Plasmid#192379PurposeTo test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3A
Plasmid#198174PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitution in codon 225PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3B
Plasmid#198175PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastMutationPromoteryeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-OsAct2-11
Plasmid#197765PurposePhytobrick (MoClo) Level 0 PartDepositorInsertOsAct2 terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
lvl0 ACT2p
Plasmid#153374PurposePlant promoterDepositorInsertACT2 promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMYA5/pACT2
Plasmid#97208PurposeCMYA5 (aa 4039 – 4069) in a GAL4 AD vector, used for yeast-two hybridDepositorInsertCMYA5 (CMYA5 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
LHP599 - pACT2-Ub
Plasmid#32170DepositorUseTagsGal4 AD and HAExpressionYeastMutationPromoterpADH1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMYA5tnT/pACT2
Plasmid#98126PurposeCMYA5 (aa 3859 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybridDepositorInsertCMYA5 (CMYA5 Human)
UseTagsExpressionYeastMutationrs10043986 (NP_705838.3:p.Pro4063Leu)PromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACT2-AP-3 σ3A
Plasmid#198347PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-AP-3 σ3B
Plasmid#198348PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ1A
Plasmid#198168PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 μ1A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+AtAct2t
Plasmid#170882PurposePhytobrick - AtACT2tDepositorInsertAtACT2t
UseTagsExpressionPlantMutation426C>T to remove BbsI restriction sitePromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 ACT2t
Plasmid#153384PurposePlant terminatorDepositorInsertACT2 terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Dhr1
Plasmid#100003PurposeExpress yeast Dhr1 with Y2H activation domain fusionDepositorInsertDhr1 (ECM16 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Mrt4
Plasmid#99998PurposeExpress yeast Mrt4 with Y2H activation domain fusionDepositorInsertMrt4 (MRT4 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Rcl1
Plasmid#99992PurposeExpress yeast Rcl1 with Y2H activation domain fusionDepositorInsertRcl1 (RCL1 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationContains amino acids 1-217 of Rcl1PromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Kre33
Plasmid#99990PurposeExpress yeast Kre33 with Y2H activation domain fusionDepositorInsertKre33 (KRE33 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Esf1
Plasmid#99988PurposeExpress yeast Esf1 with Y2H activation domain fusionDepositorInsertEsf1 (ESF1 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Utp15
Plasmid#99978PurposeExpress yeast Utp15 with Y2H activation domain fusionDepositorInsertUtp15 (UTP15 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMYA5tnC/pACT2
Plasmid#98125PurposeCMYA5 (aa 3859 – 4069) in a GAL4 AD vector, used for yeast-two hybridDepositorInsertCMYA5 (CMYA5 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMYA5T/pACT2
Plasmid#97209PurposeCMYA5 (aa 4039 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybridDepositorInsertCMYA5 (CMYA5 Human)
UseTagsExpressionYeastMutationrs10043986 (NP_705838.3:p.Pro4063Leu)PromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTAct2 (GB0210)
Plasmid#68191PurposeProvides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid partDepositorInsertAtAct2 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only